{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:04:23Z","timestamp":1790798663110,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T00:00:00Z","timestamp":1744156800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071328"],"award-info":[{"award-number":["82071328"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Subarachnoid hemorrhage (SAH) is one of the most devastating hemorrhagic strokes. SAH causes neuroinflammation and leads to both early brain injury and delayed brain injury. G\u2010protein\u2010coupled receptor 84 (GPR84), one of the orphan class\u2010A G protein\u2010coupled receptors (GPCRs), exerts pro\u2010inflammatory and pro\u2010phagocytic effects via targeting microglia in the central nervous system (CNS). This research investigated the role of GPR84 on SAH pathology via neuroinflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>An enzyme\u2010linked immunosorbent assay was used for GPR84 expression in cerebrospinal fluid (CSF) samples from patients with SAH. An experimental SAH\u2010model mouse was established by stereotactic injection of autologous blood into the chiasmatic cisterna. The SAH model in vitro was established by exposing microglia to hemoglobin. After inhibition of GPR84 in mice by GPR84\u2010siRNA and GPR84\u2010antagonist 3, the neurological deficits were evaluated by modified Garcia test, beam balance test, and Morris water maze. Neuronal death in SAH\u2010model mice was evaluated by Nissl staining. GPR84, NLRP3 inflammasome, and cAMP\/PKA expressions were detected by western blot and immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>GPR84 was upregulated in patients after SAH onset. The GPR84 expression in microglia increased after SAH onset, activated NLRP3 inflammasome, and promoted IL\u20101\u03b2 secretion. Both GPR84\u2010shRNA and GPR84\u2010antagonist 3 improved neurological deficits in SAH\u2010model mice. Mechanistically, GPR84 activated the NLRP3 inflammasome via the cAMP\/PKA signaling pathway to aggravate neuronal injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>GPR84 promotes NLRP3\u2010mediated pyroptosis and activated NLRP3 inflammation via cAMP\/PKA pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.70379","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T03:24:05Z","timestamp":1744169045000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["G\u2010Protein\u2010Coupled Receptor 84 Aggravates Early Brain Injury via Microglial NLRP3\u2010ASC Inflammasome After Subarachnoid Hemorrhage"],"prefix":"10.1002","volume":"15","author":[{"given":"Kun","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Jinling 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