{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:54:50Z","timestamp":1791899690250,"version":"4.3.4"},"reference-count":94,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T00:00:00Z","timestamp":1741478400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Insufficient sleep and insomnia are common issues associated with modern lifestyles that often contribute to the development of mental health disorders. 4\u2010aminopyridine (4\u2010AP), a voltage\u2010gated potassium (Kv) channel antagonist, is commonly used in the treatment of multiple sclerosis (MS). It has been shown to improve nerve conduction velocity, strengthen myelin, and increase axonal area after injury. In addition, 4\u2010AP has been reported to reduce behavioral disorders, including depression. The aim of this study was to investigate the effects of 4\u2010AP on anxiety\u2010like behavior in mice subjected to rapid eye movement (REM) sleep deprivation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifty male mice were randomly divided into five groups: control, normal saline (NS) (receiving normal saline via gavage), AP\u20100.25, AP\u20100.5, and AP\u20101 (receiving daily doses of 0.25, 0.5, and 1\u00a0mg\/kg of 4\u2010AP, respectively by gavage). All groups except the control group underwent SD for five consecutive days. The animals' locomotion and anxiety\u2010like behavior were assessed using the open field and elevated plus maze tests. After behavioral testing, N\u2010methyl\u2010D\u2010aspartate receptor (NMDA\u2010R), \u03b1\u2010amino\u20103\u2010hydroxy\u20105\u2010methyl\u20104\u2010isoxazolepropionic acid receptor (AMPA\u2010R), and tumor necrosis factor (TNF\u2010\u03b1) were measured by western blotting, and also malondialdehyde (MDA) and total antioxidant capacity (TAC) were analyzed by ELISA in the hippocampus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Finding<\/jats:title>\n                    <jats:p>AP\u20101 significantly reduced the levels of anxiety\u2010like behavior compared to the NS group in both tests. In AP\u20101, a significant decrease in the levels of NMDA\u2010R, AMPA\u2010R, TNF\u2010\u03b1, and MDA was observed. While these levels were increased in the NS group. In addition, AP\u20101 showed a higher level of TAC compared to the NS group, indicating an increase in antioxidant levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>4\u2010AP may be effective in reducing anxiety\u2010like behavior in sleep\u2010deprived mice by modifying the levels of NMDA\u2010R, AMPA\u2010R, and TNF\u2010\u03b1, while simultaneously reducing oxidative stress induced by sleep deprivation in the hippocampus.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.70382","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T18:35:34Z","timestamp":1741718134000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sleep Deprivation\u2010Induced Anxiety Alleviated by Oral Administration of 4\u2010Aminopyridine in Male 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