{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:00:54Z","timestamp":1772118054883,"version":"3.50.1"},"reference-count":80,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T00:00:00Z","timestamp":1741824000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Individuals with autistic spectrum disorders (ASD) primarily exhibit deficits in communication and social interaction, along with repetitive behaviors and restricted interests. This disorder is often associated with anxiety, nociceptive disorders, and pain. While medical treatment generally focuses on treating the symptoms rather than addressing the underlying causes, traditional medicine is sometimes used as an alternative.\n                      <jats:italic>Piptadeniastrum africanum<\/jats:italic>\n                      is used in Cameroonian medicinal folks to treat cognitive disorders. However, its effects and mechanisms of action regarding the inhibition of ASD\u2010like symptoms remain unclear. The primary goal of the present study was to evaluate the anxiolytic and analgesic effects of the water extract of\n                      <jats:italic>P. africanum<\/jats:italic>\n                      on autistic triad induced in rats by sodium valproate.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      The study investigated the secondary metabolites in\n                      <jats:italic>P. africanum<\/jats:italic>\n                      extract using UHPLC\u2010MS. DPPH, ABTS, and FRAP tests were performed to assess the extract's ability to neutralize free radicals. Molecular docking was utilized to evaluate the extract's binding to various receptors. For the experimental study, 33 pregnant female rats were divided into two groups after pregnancy was confirmed. One group was given distilled water orally at 10\u00a0mL\/kg, while the other group received sodium valproate at 800\u00a0mg\/kg on gestation days 11, 12, and 13. When the male offspring reached 3 weeks old, they were evaluated for anxiety, social interaction, and pain sensitivity, with those displaying any disorders selected for further study. The remaining rats were split into six groups of five and treated with either a vehicle, bumetanide, or\n                      <jats:italic>P. africanum<\/jats:italic>\n                      extract at 190 and 760\u00a0mg\/kg. Behavioral assessments focusing on sociability, anxiety, and pain sensitivity were conducted on days 28 and 37 after weaning. In the end, biochemical markers related to GABA metabolism, serotonin levels, and oxidative status were analyzed in the cerebellum, prefrontal cortex, hippocampus, and amygdala alongside histopathological analyses in the brain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      UHPLC\u2010MS allows us to identify several compounds. They bind to H3R (7F61) and HDAC2 through conventional hydrogen bonding. Findings showed that prenatal administration of sodium valproate induced in male offspring a deficit in social interaction (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), anxiety disorders (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), hypersensitivity to pain (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), increased GABA and serotonin concentration (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), disturbed oxidative status (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001), and neuronal loss (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001) as well as neuronal disorganization in the hippocampus, cerebellum and amygdala in young rats compared to neurotypical animals.\n                      <jats:italic>P. africanum<\/jats:italic>\n                      extract at doses used, like bumetanide, corrected these disorders and protected against neuronal loss. These results suggest that the extract has anxiolytic and anti\u2010nociceptive effects. It has been found that the positive effects can be achieved by restoring GABAergic and serotonergic neurotransmission, coupled with antioxidant and neuromodulatory activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The current findings support that\n                      <jats:italic>P. africanum<\/jats:italic>\n                      induces anxiolytic and analgesic effects in a sodium valproate\u2010induced autistic disorders model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.70408","type":"journal-article","created":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T08:15:39Z","timestamp":1741853739000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phytochemical, In Silico, In Vitro, and In Vivo Research on\n                    <i>Piptadeniastrum africanum<\/i>\n                    (Fabaceae) Unveiling Anti\u2010Stereotypic, Anxiolytic, and Analgesic Effects in a Sodium Valproate\u2010Induced Autistic Disorders Model"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1239-2907","authenticated-orcid":false,"given":"Ambani Omgba Jeanne","family":"Julie","sequence":"first","affiliation":[{"name":"Department of Animal Biology and Physiology, Laboratory of Animal Physiology, Faculty of Science University of Yaound\u00e9 1  Yaound\u00e9 Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4586-426X","authenticated-orcid":false,"given":"Ngouateu Omer","family":"B\u00e9b\u00e9","sequence":"additional","affiliation":[{"name":"Department of Animal Biology and Physiology, Laboratory of Animal Physiology, Faculty of Science University of Yaound\u00e9 1  Yaound\u00e9 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