{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T16:00:54Z","timestamp":1792684854495,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Alzheimer's disease (AD) is a leading cause of disability worldwide. Alterations in cerebral blood flow (CBF) and AD blood biomarkers are fundamental at early stages of AD. Exercise shows promise in delaying physiological changes, but its mechanisms for enhancing brain health remain unclear. flADex aims to examine the acute effects of different exercise types on CBF and blood biomarkers in older adults. This protocol describes the methodology and rationale of flADex.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>flADex is a counterbalanced crossover trial in 20 older adults, aged 68\u201383\u00a0years old, with negative brain amyloid status (&lt;\u00a012 centiloid) who are APOE\u03b54 noncarriers. Participants will complete a 30\u2010min session of each condition in a randomized order: (i) moderate\u2010intensity aerobic exercise (60%\u201370% age\u2010predicted maximal heart rate), (ii) moderate\u2010intensity resistance exercise (rating of perceived exertion: 4\u20136 points out of 10), and (iii) resting condition. Changes in CBF are the primary outcome and will be assessed by magnetic resonance imaging using pseudo\u2010continuous arterial spin labeling at pre\u2010 and at 3 timepoints post\u2010condition (starting at 20, 27, 34\u00a0min). Secondary outcomes are biomarkers of AD pathology and neurodegeneration (A\u03b242, A\u03b240, p\u2010tau217, p\u2010tau181, BD\u2010tau, GFAP, NfL) and growth factors (BDNF, IGF\u20101), measured through blood samples collected at pre\u2010 and post\u2010condition (at 3, 50, 70\u00a0min). Moreover, cognitive outcomes and mood status will be measured pre\u2010 and post\u2010condition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>flADex will highlight the acute effects of different\u00a0exercise types on CBF and biomarkers before beta\u2010amyloid accumulation. Acute effects on CBF dynamics and blood biomarkers are expected to be greater with aerobic than resistance exercise when compared to resting. CBF is expected to vary by brain region, and biomarkers to fluctuate dynamically postexercise. This will provide critical insights into exercise's impact on vascular and molecular pathways associated with AD pathology and potential recommendations for standardized blood sampling to enhance diagnostic accuracy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.70636","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T19:25:41Z","timestamp":1761938741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Understanding Cerebral Blood Flow Dynamics for Alzheimer's Disease Prevention Through Acute Exercise (flADex): Protocol for a Randomized Crossover Trial"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4268-1935","authenticated-orcid":false,"given":"Isabel","family":"Mart\u00edn\u2010Fuentes","sequence":"first","affiliation":[{"name":"Department of Physical and Sports Education, Sport and Health University Research Institute 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