{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T06:40:15Z","timestamp":1758868815517,"version":"3.44.0"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This research focused on investigating acupuncture's effect on brain injury in middle cerebral artery occlusion (MCAO) rats and elucidating its potential mechanisms, with a focus on transcriptomic and protein\u2010level changes related to the complement system, which may be involved in acupuncture's therapeutic effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An MCAO rat model was established and treated with acupuncture. Brain tissue from the infarct area was analyzed through RNA sequencing, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein\u2013protein interaction (PPI) analyses, followed by qRT\u2010PCR and western blot validation of key complement\u2010related genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 1792 differentially expressed genes (DEGs) were identified between the MCAO and acupuncture groups. Of these, 254 hub genes were associated with acupuncture's therapeutic effects. Seven complement\u2010related genes (C1QA, C1QB, C1QC, C2, C6, C7, and TREM2) were significantly downregulated by acupuncture. Functional enrichment revealed that these genes were involved in the inflammatory response, coagulation cascades, blood\u2012brain barrier regulation, and neutrophil extracellular trap (NET) formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that acupuncture alleviates stroke\u2010induced brain injury by modulating key components of the complement system and associated inflammatory pathways. These results provide mechanistic insights supporting the use of acupuncture as an adjuvant therapy for poststroke neuroinflammation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.70740","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T05:32:48Z","timestamp":1755840768000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Transcriptomic Analysis of Brain Tissues From the Infarcted Area of MCAO Rats Revealed That Acupuncture Attenuates Brain Injury via the Complement System"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6076-7998","authenticated-orcid":false,"given":"Yanlin","family":"Liu","sequence":"first","affiliation":[{"name":"Acupuncture and Moxibustion Department Jiaxing Hospital of Traditional Chinese Medicine Affiliated With Zhejiang University of Traditional Chinese Medicine  Jiaxing 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