{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T15:55:08Z","timestamp":1786636508900,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271572"],"award-info":[{"award-number":["82271572"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82022023"],"award-info":[{"award-number":["82022023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82401774"],"award-info":[{"award-number":["82401774"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017596","name":"Natural Science Basic Research Program of Shaanxi Province","doi-asserted-by":"publisher","award":["S2024\u2010JC\u2010QN\u20102742"],"award-info":[{"award-number":["S2024\u2010JC\u2010QN\u20102742"]}],"id":[{"id":"10.13039\/501100017596","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study investigates the efficacy of acupuncture at Baihui (GV20) and Zusanli (ST36) acupoints in alleviating depressive symptoms and elucidates the underlying mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sixty male C57BL\/6J mice were subjected to an 8\u2010week chronic unpredictable mild stress (CUMS) paradigm. Acupuncture was administered to two groups during the final 3 weeks: one under CUMS and the other without stress. Behavioral assessments, including the sucrose preference test and tail suspension test (TST), evaluated depression\u2010like behaviors. 16S rRNA sequencing and non\u2010targeted metabolomics analyzed gut microbiota and metabolites, respectively, with association analysis exploring the mechanistic pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Acupuncture significantly ameliorated CUMS\u2010induced depression\u2010like behaviors, restoring sucrose preference (from a significant reduction in CUMS,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001, to an increase in CUMS\u00a0+\u00a0AP,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.035) and reducing immobility time in the TST (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.045). 16S rRNA sequencing revealed that acupuncture partially restored the CUMS\u2010disrupted\n                      <jats:italic>Bacteroidetes<\/jats:italic>\n                      \/\n                      <jats:italic>Firmicutes<\/jats:italic>\n                      ratio by specifically decreasing the pathobiont\n                      <jats:italic>Clostridium<\/jats:italic>\n                      sp.\n                      <jats:italic>A3LF 105b<\/jats:italic>\n                      and increasing beneficial bacteria such as\n                      <jats:italic>Faecalibacterium<\/jats:italic>\n                      and\n                      <jats:italic>Lachnospiraceae UCG 001<\/jats:italic>\n                      . These microbial shifts were functionally linked to the elevation of the critical metabolite sphinganine 1\u2010phosphate (S1P). Spearman correlation analysis revealed a negative correlation between\n                      <jats:italic>Clostridium<\/jats:italic>\n                      sp.\n                      <jats:italic>A3LF 105b<\/jats:italic>\n                      and S1P (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01,\n                      <jats:italic>R<\/jats:italic>\n                      \u00a0&lt;\u00a0\u22120.52). The modulation of gut microbiota led to increased S1P, which in turn activated the \u201cneuroactive ligand\u2013receptor interaction\u201d pathway, thereby restoring neuronal activity and alleviating depressive behavior.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study underscores the potential of acupuncture as an antidepressant treatment, highlighting its impact on gut microbiota and metabolic pathways in alleviating depressive symptoms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.71022","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T06:18:18Z","timestamp":1761805098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Acupuncture for Major Depressive Disorder: Exploring the Gut Microbiota as a Novel Therapeutic Pathway"],"prefix":"10.1002","volume":"15","author":[{"given":"Cailing","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Key Laboratory of Biological Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Key Laboratory of Biological Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarong","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Department of Psychiatry the Ankang Central Hospital  Ankang Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pu","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Key Laboratory of Biological Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Key Laboratory of Biological Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Key Laboratory of Biological Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Key Laboratory of Biological Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya'ni","family":"Yang","sequence":"additional","affiliation":[{"name":"Xi'an Mental Health Center Xi'an Shannxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Department of Neurology the First People's Hospital of Xianyang  Xianyang Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6532-5469","authenticated-orcid":false,"given":"Ruina","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi China"},{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Shaanxi Provincial Key Laboratory of Biological Psychiatry The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0ra05326k"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2022.101300"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201034504\u20101"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2023.115383"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2019.08.034"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.83079"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2024.10421"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.106751"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuad014"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001738\u2010y"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2023.45138"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.adi5374"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2025.2514138"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.12.029"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118582"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010021\u201001123\u20105"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbab110"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010024\u201001756\u20106"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1777"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2023.2271620"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2019.09.022"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.20230382"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2024.05.030"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010024\u201000990\u20102"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/js9.0000000000001984"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201047273\u2010w"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201004001\u20104"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2025.137158"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2024.122779"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2021.02.037"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2025.103580"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010015\u20109042\u20102"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.1.29"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.595342"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpr.2020.101943"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac051437y"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1536056"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl838"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl\u20102024\u2010332245"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2021.10.002"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673\u20105374.300454"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010020\u201000417\u20108"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.11.009"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.14260"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155584"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl923"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41386\u2010024\u201002036\u20105"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.319"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.Cd015528"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673\u20105374.286973"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba8555"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202402856"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2022.20563"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.13703\/j.0255\u20102930.20231019\u20100003"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13058\u2010023\u201001645\u20100"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.121052"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.121368"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2024.51217"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2022.02.007"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010019\u20100733\u20103"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcn.13512"}],"container-title":["Brain and Behavior"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.71022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/brb3.71022","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.71022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:57:51Z","timestamp":1786633071000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/brb3.71022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":61,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/brb3.71022"],"URL":"https:\/\/doi.org\/10.1002\/brb3.71022","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/BRB3.71022\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71022\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71022\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71022\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71022\/v1\/decision1","asserted-by":"object"}]},"ISSN":["2162-3279","2162-3279"],"issn-type":[{"value":"2162-3279","type":"print"},{"value":"2162-3279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]},"assertion":[{"value":"2025-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71022"}}