{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:18:09Z","timestamp":1792113489624,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274655"],"award-info":[{"award-number":["82274655"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82405579"],"award-info":[{"award-number":["82405579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2022\u2010JYB\u2010XJSJJ\u2010038"],"award-info":[{"award-number":["2022\u2010JYB\u2010XJSJJ\u2010038"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Muscle spasticity, a prevalent and debilitating motor impairment after stroke, severely compromises patients\u2019 functional recovery and quality of life. Motion\u2010style scalp acupuncture (MSSA), a novel integrative therapy combining scalp acupuncture (SA) with targeted exercise training, has emerged as a promising strategy for post\u2010stroke spasticity (PSS). This study investigated the mechanisms underlying MSSA's therapeutic effects, focusing on 5\u2010HT\n                      <jats:sub>2A<\/jats:sub>\n                      R\u2010mediated KCC2 reactivation in spinal motoneurons.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using a rat model of permanent middle cerebral artery (MCA) occlusion, we evaluated MSSA's effects through behavioral assessments (Zea Longa, modified Ashworth scale (MAS), screen test), cerebral blood flow (laser speckle imaging), and infarct area analysis (TTC staining). Spinal reflex excitability was quantified via H\u2010reflex electrophysiological recordings, while molecular mechanisms were examined using Western blot, RT\u2010qPCR, and double immunofluorescence labeling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our results demonstrated that MSSA significantly improved neurological function, reduced muscle tone, and attenuated spinal hyperreflexia. These improvements were associated with enhanced cerebral blood flow and reduced infarct areas. Furthermore, MSSA significantly upregulated the expression of 5\u2010HT\n                      <jats:sub>2A<\/jats:sub>\n                      R and KCC2 in the spinal cord. Pharmacological validation using a 5\u2010HT\n                      <jats:sub>2A<\/jats:sub>\n                      R antagonist and agonist confirmed the crucial role of 5\u2010HT\n                      <jats:sub>2A<\/jats:sub>\n                      R in mediating MSSA's therapeutic effects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings demonstrate that MSSA ameliorates PSS through dual mechanisms: improving cerebral blood flow to the ischemic penumbra and restoring spinal inhibitory control via 5\u2010HT\n                      <jats:sub>2A<\/jats:sub>\n                      R\u2010dependent KCC2 reactivation. This study not only elucidates the mechanisms of MSSA but also provides a novel therapeutic strategy for PSS management.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.71064","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T05:51:16Z","timestamp":1764222676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Motion\u2010Style Scalp Acupuncture Ameliorates Post\u2010Stroke Muscle Spasticity Through Cerebral Blood Flow Augmentation and 5\u2010HT\n                    <sub>2A<\/sub>\n                    R\u2010Mediated Spinal KCC2 Reactivation"],"prefix":"10.1002","volume":"15","author":[{"given":"Qin\u2010Yong","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion and Tuina Beijing University of Chinese Medicine  Chaoyang District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0927-2195","authenticated-orcid":false,"given":"Jun\u2010Xiang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Nursing Beijing University of Chinese Medicine  Chaoyang District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang\u2010Xiao","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Acupuncture\u2010Moxibustion and Tuina Beijing University of Chinese Medicine  Chaoyang District Beijing China"},{"name":"The Key Unit of State Administration of Traditional Chinese Medicine Evaluation of Characteristic Acupuncture Therapy  Chaoyang District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Shan","family":"Zhong","sequence":"additional","affiliation":[{"name":"School of Nursing Beijing University of Chinese Medicine  Chaoyang District Beijing 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