{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T11:03:27Z","timestamp":1787223807327,"version":"build-2736575974"},"reference-count":68,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Introduction<\/jats:bold>\n                    : Peppermint essential oil (PEO) and its main component, menthol, are used in Western and Eastern traditional medicine for their anti\u2010spasmodic, anti\u2010septic, or anti\u2010pruritic properties. Although topical application of PEO exhibits anti\u2010pruritic efficacy, the effects of PEO inhalation on itch sensation and pruritic behavior remain unclear. We aimed to determine whether PEO inhalation alleviates pruritus and itch\u2010responsive neural activity in the brains of mice with hapten\u2010induced dermatitis under acute stress conditions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods<\/jats:bold>\n                    : Forty\u2010one female BALB\/c mice were randomly assigned to six experimental groups. Twenty\u2010nine mice were subjected to oxazolone (OXA)\u2010induced dermatitis through an initial sensitization followed by three rounds of topical application of OXA every other day. During the final OXA application, twenty\u2010two mice were exposed to restraint stress for 2\u00a0h. Sixteen mice were subjected to the inhalation of 2.5% PEO. The total duration of scratching bouts and the number of c\u2010Fos\u2010positive cells in the parabrachial nucleus, central amygdala, periaqueductal gray, and ventral tegmental area were quantified.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    : PEO inhalation reduced the duration of scratching behavior induced by the combination of repeated OXA application and acute restraint stress. The c\u2010Fos\u2010positive cell number in the tested brain regions, except the ventral tegmental area, was positively correlated with the pruritic response. PEO inhalation alleviates OXA\u2010 or stress\u2010induced pruritus by modulating neuronal activity in itch\u2010related brain regions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    : Acute restraint stress exacerbates itch, and PEO inhalation alleviates the stress\u2010associated aggravation of pruritus caused by OXA\u2010induced dermatitis, which is associated with the modulation of neuronal activity in itch\u2010related brain regions.\n                  <\/jats:p>","DOI":"10.1002\/brb3.71072","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T06:29:16Z","timestamp":1763447356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Single Inhalation of Peppermint Essential Oil Alleviates Acute Restraint Stress\u2010Exacerbated Itch in Oxazolone\u2010Induced Mild Dermatitis: Correlations With Brain Neuronal Activity in Female BALB\/c Mouse"],"prefix":"10.1002","volume":"15","author":[{"given":"Shotaro","family":"Shoji","sequence":"first","affiliation":[{"name":"Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences Fukuoka University  Jonan\u2010ku Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences Fukuoka University  Jonan\u2010ku Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hikari","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences Fukuoka University  Jonan\u2010ku Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reika","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Pharmacotherapeutics, Faculty of 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