{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:59:09Z","timestamp":1791561549186,"version":"4.3.4"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007446","name":"King Khalid University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007446","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Insomnia and other sleep disorders are becoming increasingly prevalent worldwide, while current sedative medications such as benzodiazepines, though effective, are often limited by side effects and dependency risks. Therefore, identifying safe natural compounds with sedative potential is of growing scientific and clinical interest. L\u2010quebrachitol (LQL), a naturally occurring cyclitol compound with antioxidant, antimicrobial, and antidiabetic properties, has not been previously evaluated for its sedative effects. The aim of this study is to evaluate the potential sedative effects of LQL through both in vivo and in silico methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this experiment, 2\u2010day\u2010old broiler chicks (\n                      <jats:italic>Gallus gallus domesticus<\/jats:italic>\n                      ) were given thiopental sodium (10\u00a0mg\/kg, intraperitoneal [ip]) to induce sleep. LQL (1, 5, and 10\u00a0mg\/kg, ip) and diazepam (2\u00a0mg\/kg, ip) were administered alone or together to assess their synergistic or antagonistic effects on chicks. To assess its potential for interacting with the GABA\n                      <jats:sub>A<\/jats:sub>\n                      receptor (\u03b11 and \u03b22 subunits), a molecular docking study was carried out.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      According to the in vivo investigation, the results indicate that LQL decreased the latency period while extending the animal's sleep duration time in a dose\u2010dependent manner. Moreover, the combination of LQL\u201010 (10\u00a0mg\/kg) and diazepam 2 (2\u00a0mg\/kg) showed (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) enhanced sedative effects significantly by decreasing latency time and prolonging sleeping duration. In addition, LQL has a moderate binding affinity of \u22125.3\u00a0kcal\/mol against the GABA\n                      <jats:sub>A<\/jats:sub>\n                      receptor (\u03b11 and \u03b22 subunits), but forms strong hydrogen bond interactions and similar amino acid residues with standard drug diazepam, suggesting potential therapeutic effects. Further, LQL also demonstrated promising pharmacokinetic properties and low toxicity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings collectively enhance the potential of LQL as an effective sedative therapeutic agent. However, further research, including in vitro studies to confirm the molecular interactions and membrane permeability, followed by well\u2010designed clinical trials, is necessary to fully establish LQL as a safe and effective sedative agent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.71185","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T08:35:59Z","timestamp":1768293359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["L\u2010Quebrachitol Enhances Sedative Effect of Diazepam Through GABAergic Pathway: Animal and Computational Studies"],"prefix":"10.1002","volume":"16","author":[{"given":"Asifa","family":"Asrafi","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Gopalganj Science and Technology University  Gopalganj Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Aslam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Gopalganj Science and Technology University  Gopalganj Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Sakib","family":"Al Hasan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University  Gopalganj Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  Gopalganj Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Burhan","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, York College City University of New York  Jamaica New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emon","family":"Mia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University  Gopalganj Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  Gopalganj Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Y.","family":"Alshahrani","sequence":"additional","affiliation":[{"name":"Central Labs King Khalid University  Abha Saudi Arabia"},{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences King Khalid University  Abha Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumaya Akter","family":"Bithi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Gopalganj Science and Technology University  Gopalganj Bangladesh"},{"name":"Bioinformatics and Drug Innovation Laboratory BioLuster Research Center Ltd.  Gopalganj Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mst. 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