{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T09:02:36Z","timestamp":1780909356983,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the effects of An Shen Jie Yu Tang (ASJYD) on hippocampal morphology, AKT\/mTOR phosphorylation, and serum adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels in rats subjected to chronic unpredictable mild stress (CUMS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Three groups were used: Control, CUMS, and CUMS+ASJYD. Over 30 days, the CUMS and CUMS+ASJYD groups were exposed to stressors. After stress induction and behavioral assessments, the CUMS+ASJYD group received ASJYD for 14 days. Serum ACTH and CORT were quantified by ELISA. Hippocampal p\u2010AKT\/p\u2010mTOR protein expression was evaluated by Western blot. Hippocampal neuron morphology was examined via Nissl staining, and mitochondrial ultrastructure via transmission electron microscopy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared to controls, CUMS rats showed reduced sucrose preference, decreased distance\/time in the open field center, and increased water maze latency (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01). ASJYD ameliorated these impairments (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01). CUMS rats exhibited elevated ACTH\/CORT and decreased p\u2010AKT\/p\u2010mTOR (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01). ASJYD reduced ACTH\/CORT and increased p\u2010AKT\/p\u2010mTOR (\n                      <jats:italic>p<\/jats:italic>\n                      &lt;0.01). Histology revealed CUMS rats had disorganized hippocampal neurons, reduced cell numbers, and nuclear abnormalities, which were improved by ASJYD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ASJYD ameliorates CUMS\u2010induced behavioral deficits, reduces hypothalamic\u2010pituitary\u2010adrenal axis hyperactivity, is associated with increased phosphorylation of AKT and mTOR, and protects against hippocampal neuronal damage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.71380","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T04:40:39Z","timestamp":1775018439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anshen Jieyu Decoction: A Promising Remedy for Depression via AKT\/mTOR Pathway Modulation in CUMS Rats"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8828-1033","authenticated-orcid":false,"given":"Zhen","family":"Li","sequence":"first","affiliation":[{"name":"Undergraduate Academic Affairs Office Qilu Medical University  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2719-1888","authenticated-orcid":false,"given":"Zijun","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Medical Imaging Qilu Medical University  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7846-3032","authenticated-orcid":false,"given":"Zehao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Clinical Medicine Qilu Medical University  Zibo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuzhang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Medical Imaging 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