{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T09:49:32Z","timestamp":1791625772019,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Corpora amylacea<\/jats:italic>\n                      (CA) are starch\u2010like inclusions that accumulate in the central nervous system (CNS) with aging and are enriched in neurodegenerative conditions, including amyotrophic lateral sclerosis (ALS). Although often regarded as waste reservoirs, their cellular origins, molecular composition, and pathological significance remain poorly understood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here, we performed an unbiased proteomic analysis of purified CAs isolated from\n                      <jats:italic>post\u2010mortem<\/jats:italic>\n                      brains of sporadic ALS patients and controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings<\/jats:title>\n                    <jats:p>In\u2010depth mass spectrometry identified 4,470 proteins, of which 658 were quantified, revealing distinct ALS\u2010specific proteomic signatures. Enriched proteins included markers of cytoskeletal remodeling, mitochondrial dysfunction, and proteostasis disruption, as well as known ALS\u2010associated proteins such as TDP\u201043 and neurofilament proteins. These findings demonstrate that CAs serve as reservoirs of dysfunctional, disease\u2010relevant proteins and capture key pathological processes in ALS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>By applying an unbiased proteomic approach to purified CAs, this study provides the first comprehensive map of their protein content in ALS, supporting their potential as biomarker sources and as a source of mechanistic insights into neurodegeneration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance<\/jats:title>\n                    <jats:p>Unbiased analyses of CAs in the context of ALS have yet to be undertaken. This study provides the first proteomic profiling of purified CAs, isolated from ALS patient brains using biochemical methods, revealing that CAs harbor disease\u2010relevant proteins implicated in sporadic ALS. By demonstrating that CAs act as reservoirs of dysfunctional proteins related to metabolism, cytoskeletal organization, and proteostasis, our findings highlight their potential as a novel source of ALS\u2010specific mechanistic insight into disease pathology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.71486","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T05:02:08Z","timestamp":1779685328000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic Analysis of\n                    <i>Corpora Amylacea<\/i>\n                    Extracted From\n                    <i>Post\u2010mortem<\/i>\n                    Brain of MAiD\u2010end\u2010of\u2010life Sporadic ALS Patients"],"prefix":"10.1002","volume":"16","author":[{"given":"Alexandre","family":"Paquet","sequence":"first","affiliation":[{"name":"Department of Surgery, Faculty of Medicine Universit\u00e9 Laval  Quebec City QC Canada"},{"name":"Regenerative Medicine Research Program CHU De Qu\u00e9bec\u2010Universit\u00e9 Laval Research Center  Qu\u00e9bec QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Touzel\u2010Desch\u00eanes","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine Universit\u00e9 Laval  Quebec City QC Canada"},{"name":"Regenerative Medicine Research Program CHU De Qu\u00e9bec\u2010Universit\u00e9 Laval Research Center  Qu\u00e9bec QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine Universit\u00e9 Laval  Quebec City QC Canada"},{"name":"Regenerative Medicine Research Program CHU De Qu\u00e9bec\u2010Universit\u00e9 Laval Research Center  Qu\u00e9bec QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Saikali","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Division of Anatomic Pathology and Neuropathology CHU De Qu\u00e9bec  Quebec City QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Dupr\u00e9","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Medicine Laval University  Quebec City QC Canada"},{"name":"Neuroscience Research Program CHU De Qu\u00e9bec\u2010Universit\u00e9 Laval Research Center  Qu\u00e9bec QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3825-4885","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Gros\u2010Louis","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine Universit\u00e9 Laval  Quebec City QC Canada"},{"name":"Regenerative Medicine Research Program CHU De Qu\u00e9bec\u2010Universit\u00e9 Laval Research Center  Qu\u00e9bec QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010024\u201001812\u20100"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201004199\u20103"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00691318"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201038010\u20107"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41807"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201031766\u2010y"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23463"},{"issue":"5","key":"e_1_2_14_9_1","first-page":"550","article-title":"Corpora Amylacea of the Optic Nerve and Retina: a Form of Neuronal Degeneration","volume":"19","author":"Avendano J.","year":"1980","journal-title":"Investigative ophthalmology & visual science"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0165\u20100173(99)00003\u2010x"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp\u20102020\u2010322983"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.181648"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddw180"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaf1059"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1474\u20104422(10)70195\u20102"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/ene.14393"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv468"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0341261"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201031773\u2010z"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010014\u20100181\u2010z"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.12974\/2309\u20106128.2014.02.01.6"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201028231\u20101"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2016.00086"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1913741116"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101484"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2211326119"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010023\u201002664\u20105"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.23937\/2378\u20103001\/2\/2\/1031"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2008_3199"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac194"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097\u20100134(199707)28:3<405::aid\u2010prot10>3.0.co;2\u2010l"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010024\u201001866\u20100"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2009.04.019"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010025\u201005085\u2010z"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.705594"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201009234\u20106"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr060161n"}],"container-title":["Brain and Behavior"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.71486","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/brb3.71486","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.71486","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T09:07:06Z","timestamp":1791623226000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/brb3.71486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/brb3.71486"],"URL":"https:\/\/doi.org\/10.1002\/brb3.71486","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/BRB3.71486\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v3\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.71486\/v1\/review1","asserted-by":"object"}]},"ISSN":["2162-3279","2162-3279"],"issn-type":[{"value":"2162-3279","type":"print"},{"value":"2162-3279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2025-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71486"}}