{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:00:04Z","timestamp":1791460804121,"version":"4.3.4"},"reference-count":84,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Chronic stress exacerbates anxiety and depression, prompting interest in adaptogens that enhance resilience via redox modulation. Building on its established systemic antioxidant properties and traditional use as a tonic, we hypothesized that flavonoid\u2010rich\n                      <jats:italic>Bauhinia forficata<\/jats:italic>\n                      leaves possess unexplored adaptogenic\u2010like potential in the central nervous system. Therefore, this study aimed to assess the adaptogenic\u2010like potential of crude extracts (CEs) and dichloromethane (DCM) fraction from\n                      <jats:italic>B. forficata leaves<\/jats:italic>\n                      in an acute stress mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Adult male C57BL\/6J\/UFU mice were divided into two independent experimental sets. The first set evaluated the CE (five groups [\n                      <jats:italic>n<\/jats:italic>\n                      = 8\u20139 per group]: naive, stress plus vehicle, and stress plus CE at 30, 100, or 300\u00a0mg\/kg). The second set evaluated the DCM fraction (five groups [\n                      <jats:italic>n<\/jats:italic>\n                      = 8\u20139 per group]: naive, stress plus vehicle, and stress plus DCM fraction at 10, 30, or 100\u00a0mg\/kg). Treatments were administered orally for 4 days, beginning 2 days before stress induction. A 12\u2010h social isolation protocol without food or water induced acute stress. Behavioral assessments included the forced swim test (depressive\u2010like behavior), elevated plus maze (anxiety\u2010like behavior), and open field test (anxiety and locomotor activity). Serum urea and alanine aminotransferase (ALT) levels assessed potential hepatic and renal damage. Redox biomarkers such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione content, ferric reducing antioxidant power, sulfhydryl groups, reactive oxygen species, and lipid peroxidation were quantified in the cortex, striatum, cerebellum, and hippocampus of four groups of mice (\n                      <jats:italic>n<\/jats:italic>\n                      = 8 per group): naive, stress plus vehicle, and stress plus CE 30\u00a0mg\/kg or DCM fraction at 10\u00a0mg\/kg, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Both CE (30\u00a0mg\/kg) and DCM (10\u00a0mg\/kg) significantly reduced immobility in the forced swim test and increased time spent in the open arms of the elevated plus maze, indicating antidepressant\u2010 and anxiolytic\u2010like effects. Higher doses displayed reduced efficacy. No significant differences in serum urea or ALT were detected. Redox assessments revealed that CE and DCM treatments restored antioxidant capacity and decreased lipid peroxidation, particularly in the striatum and cortex. CE and DCM from\n                      <jats:italic>B. forficata<\/jats:italic>\n                      leaves exhibit adaptogenic\u2010like properties, enhancing behavioral resilience and modulating oxidative stress in an acute stress mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings highlight their preventive potential against acute stress\u2010induced behavioral and oxidative changes, though further mechanistic and translational studies are needed to elucidate molecular targets and clinical applicability.<\/jats:p>\n                  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