{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:26:44Z","timestamp":1777652804054,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,30]],"date-time":"2017-12-30T00:00:00Z","timestamp":1514592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2018,1]]},"abstract":"Abstract<\/jats:title>Background and Aim<\/jats:title>Hypoxic\u2013ischemic encephalopathy (HIE<\/jats:styled-content>) is a disorder featured by hypoxic and ischemic damages during the perinatal period and its high mortality (i.e., 15%\u201320%) could be partly attributed to late diagnosis. Therefore, miR\u2010210 and miR\u2010374a were investigated to find if they could improve the diagnostic values of S100B protein and neuron\u2010specific enolase (NSE<\/jats:styled-content>) for HIE<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Altogether 167 HIE<\/jats:styled-content> newborns and 82 healthy newborns were recruited, and their blood were sampled for determining the levels of biomarkers. Specifically, S100B protein and NSE<\/jats:styled-content> levels were detected based on the enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) kit, while the expressions of miR\u2010210 and miR\u2010374a were quantified by quantitative reverse transcription\u2013polymerase chain reaction (qRT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>). Moreover, the receiver operating characteristic (ROC<\/jats:styled-content>) curves were established to assess the diagnostic values of the above biomarkers for HIE<\/jats:styled-content>. Finally, the correlation analysis between miR\u2010210\/miR\u2010374 and Neonatal Behavioral Neurological Assessment (NBNA<\/jats:styled-content>) scoring or Gesell intellectual development were also conducted.<\/jats:p><\/jats:sec>Results<\/jats:title>The levels of miR\u2010210, miR\u2010374a, S100B protein, and NSE<\/jats:styled-content> were significantly distinct between HIE<\/jats:styled-content> patients and healthy newborns (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>.05). Besides, miR\u2010210 (r<\/jats:italic>s<\/jats:sub>\u00a0=\u00a0.573), miR\u2010374a (r<\/jats:italic>s<\/jats:sub>\u00a0=\u00a0.651), NSE<\/jats:styled-content> level (r<\/jats:italic>s<\/jats:sub>\u00a0=\u00a0\u2212.622), and S100B level (r<\/jats:italic>s<\/jats:sub>\u00a0=\u00a0\u2212.55) were all, respectively, correlated with NBNA<\/jats:styled-content> scoring with statistical significance (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>.05). Furthermore, it was revealed that the combined diagnosis of miR\u2010210, miR\u2010374a, S100B protein, and NSE<\/jats:styled-content> could obtain the highest accuracy regarding pairs of mild HIE<\/jats:styled-content> versus moderate HIE<\/jats:styled-content> (AUC<\/jats:styled-content>\u00a0=\u00a00.898), moderate HIE<\/jats:styled-content> versus severe HIE<\/jats:styled-content> (AUC<\/jats:styled-content>\u00a0=\u00a00.922), mild HIE<\/jats:styled-content> versus severe HIE<\/jats:styled-content> (AUC<\/jats:styled-content>\u00a0=\u00a00.996), and HIE<\/jats:styled-content> versus control (AUC<\/jats:styled-content>\u00a0=\u00a00.960). More than that, the four molecules were also remarkably associated with Gesell intellectual development (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>.05).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>MiR\u2010210 and miR\u2010374a could help to elevate the diagnostic value and prognostic prediction of S100B protein and NSE<\/jats:styled-content> for HIE<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.835","type":"journal-article","created":{"date-parts":[[2017,12,30]],"date-time":"2017-12-30T05:08:41Z","timestamp":1514610521000},"source":"Crossref","is-referenced-by-count":29,"title":["Combined prediction of miR\u2010210 and miR\u2010374a for severity and prognosis of hypoxic\u2013ischemic encephalopathy"],"prefix":"10.1002","volume":"8","author":[{"given":"Zhansheng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neonatal Intensive Care Unit The First People's Hospital of Shangqiu Shangqiu Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6024-1701","authenticated-orcid":false,"given":"Yulu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neonatal Intensive Care Unit The First People's Hospital of Shangqiu Shangqiu Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minkun","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Neonatal Intensive Care Unit The First People's Hospital of Shangqiu Shangqiu Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neonatal Intensive Care Unit The First People's Hospital of Shangqiu Shangqiu Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neonatal Intensive Care Unit The First People's Hospital of Shangqiu Shangqiu Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.braindev.2014.04.001"},{"issue":"11","key":"e_1_2_6_3_1","first-page":"712","article-title":"Exploration the diagnostic criteria for neonatal hypoxic\u2010ischemic encephalopathy","volume":"22","author":"Chen H.","year":"2004","journal-title":"Journal of Clinical Pediatrics"},{"issue":"12","key":"e_1_2_6_4_1","first-page":"940","article-title":"Diagnose and treatment for neonatal hypoxic\u2010ischemic encephalopathy","volume":"42","author":"Chen X. 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