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Their increasing drug resistance gradually makes existing antifungal drugs ineffective. Heat stable antifungal factor (HSAF) is a novel antifungal natural product with a unique structure. However, the application of HSAF has been hampered by very low yield in the current microbial producers and from extremely poor solubility in water and common solvents. In this study, we developed an effective mode of treatment applying HSAF to superficial fungal infections. The marine\u2010derived\n                    <jats:italic>Lysobacter enzymogenes<\/jats:italic>\n                    YC36 contains the HSAF biosynthetic gene cluster, which we activated by the interspecific signaling molecule indole. An efficient extraction strategy was used to significantly improve the purity to 95.3%. Scanning electron microscopy images revealed that the Type I collagen\u2010based HSAF (Col\u2010HSAF) has a transparent appearance and good physical properties, and the in vitro sustained\u2010release effect of HSAF was maintained for more than 2\u00a0weeks. The effective therapeutic concentration of Col\u2010HSAF against superficial fungal infection was explored, and Col\u2010HSAF showed good biocompatibility, lower clinical scores, mild histological changes, and antifungal capabilities in animals with\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    keratitis and cutaneous candidiasis. In conclusion, Col\u2010HSAF is an antifungal reagent with significant clinical value in the treatment of superficial fungal infections.\n                  <\/jats:p>","DOI":"10.1002\/btm2.10304","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T03:19:44Z","timestamp":1646104784000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Developing a new treatment for superficial fungal infection using antifungal\n                    <scp>Collagen\u2010HSAF<\/scp>\n                    dressing"],"prefix":"10.1002","volume":"7","author":[{"given":"Jing","family":"Zhong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiayi","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, and Institute of Evolution &amp; Marine Biodiversity Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zuo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Dou","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, and Institute of Evolution &amp; Marine Biodiversity Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, and Institute of Evolution &amp; Marine Biodiversity Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeran","family":"Bian","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, and Institute of Evolution &amp; Marine Biodiversity Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalian","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushuang","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology, School of Pharmaceutical Sciences Shandong University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyao","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chemical Biology, School of Pharmaceutical Sciences Shandong University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangcheng","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences, and Institute of Evolution &amp; 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