{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:46:08Z","timestamp":1790984768611,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T00:00:00Z","timestamp":1655078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004844","name":"National Cheng Kung University Hospital","doi-asserted-by":"publisher","award":["NCKUH\u201011102065"],"award-info":[{"award-number":["NCKUH\u201011102065"]}],"id":[{"id":"10.13039\/501100004844","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004844","name":"National Cheng Kung University Hospital","doi-asserted-by":"publisher","award":["NCKUH\u201011004013"],"award-info":[{"award-number":["NCKUH\u201011004013"]}],"id":[{"id":"10.13039\/501100004844","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004844","name":"National Cheng Kung University Hospital","doi-asserted-by":"publisher","award":["NCKUH\u201010909013"],"award-info":[{"award-number":["NCKUH\u201010909013"]}],"id":[{"id":"10.13039\/501100004844","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST110\u20102221\u2010E\u2010005\u2010010"],"award-info":[{"award-number":["MOST110\u20102221\u2010E\u2010005\u2010010"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST110\u20102314\u2010B\u2010006\u2010096"],"award-info":[{"award-number":["MOST110\u20102314\u2010B\u2010006\u2010096"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioengineering &amp;amp; Transla Med"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Urological chronic pelvic pain syndrome (UCPPS) manifests as pelvic pain with frequent urination and has a 10% prevalence rate without effective therapy. Nanoceria (cerium oxide nanoparticles [CNPs]) were synthesized in this study to achieve potential long\u2010term pain relief, using a commonly used UCPPS mouse model with cyclophosphamide\u2010induced cystitis. Transcriptome sequencing analysis revealed that serpin family B member 2 (SerpinB2) was the most upregulated marker in mouse bladder, and SerpinB2 was downregulated with CNP pretreatment. The transcriptome sequencing analysis results agreed with quantitative polymerase chain reaction and western blot analysis results for the expression of related mRNAs and proteins. Analysis of Gene Expression Omnibus (GEO) datasets revealed that SerpinB2 was a differentially upregulated gene in human UCPPS. In vitro SerpinB2 knockdown downregulated proinflammatory chemokine expression (chemokine receptor CXCR3 and C\u2010X\u2010C motif chemokine ligand 10) upon treatment with 4\u2010hydroperoxycyclophosphamide. In conclusion, CNP pretreatment may prevent the development of UCPPS, and reactive oxygen species (ROS) scavenging and SerpinB2 downregulation may modulate the immune response in UCPPS.<\/jats:p>","DOI":"10.1002\/btm2.10346","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T02:54:46Z","timestamp":1654656886000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Therapeutic potential of nanoceria pretreatment in preventing the development of urological chronic pelvic pain syndrome: Immunomodulation via reactive oxygen species scavenging and\n                    <scp>SerpinB2<\/scp>\n                    downregulation"],"prefix":"10.1002","volume":"8","author":[{"given":"Wei\u2010Chih","family":"Lien","sequence":"first","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University  Tainan Taiwan, Republic of China"},{"name":"Department of Physical Medicine and Rehabilitation, College of Medicine National Cheng Kung University  Tainan Taiwan, Republic of China"},{"name":"Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung Hsing University  Taichung City Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Ran","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University  Taipei Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Jyun","family":"Liang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University  Taipei Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congo Tak\u2010Shing","family":"Ching","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung Hsing University  Taichung City Taiwan, Republic of China"},{"name":"Graduate Institute of Biomedical Engineering National Chung Hsing University  Taichung City Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9196-0802","authenticated-orcid":false,"given":"Chia\u2010Yih","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy, College of Medicine National Cheng Kung University  Tainan Taiwan, Republic of China"},{"name":"Institute of Basic Medical Sciences, College of Medicine National Cheng Kung University  Tainan Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010I","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology National Cheng Kung University  Tainan Taiwan, Republic of China"},{"name":"The iEGG and Animal Biotechnology Center National Chung Hsing University  Taichung City Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huei\u2010Cih","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation, College of Medicine National Cheng Kung University  Tainan Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2994-6671","authenticated-orcid":false,"given":"Feng\u2010Huei","family":"Lin","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung Hsing University  Taichung City Taiwan, Republic of China"},{"name":"Institute of Biomedical Engineering, College of Medicine and College of Engineering National Taiwan University  Taipei Taiwan, Republic of China"},{"name":"Institute of Biomedical Engineering and Nanomedicine National Health Research Institutes  Zhunan, Miaoli Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4692-4917","authenticated-orcid":false,"given":"Hui\u2010Min David","family":"Wang","sequence":"additional","affiliation":[{"name":"Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung Hsing University  Taichung City Taiwan, Republic of China"},{"name":"Graduate Institute of Biomedical Engineering National Chung Hsing University  Taichung City Taiwan, Republic of China"},{"name":"Graduate Institute of Medicine, College of Medicine Kaohsiung Medical University  Kaohsiung Taiwan, Republic of China"},{"name":"Department of Medical Laboratory Science and Biotechnology China Medical University  Taichung City Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2009.08.020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000002248"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2015.03.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585\u2010018\u20100135\u20105"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2017.03.132"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/1744806918777401"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079751"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0090\u20104295(97)00179\u20109"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.01.086"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25194407"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.02.030"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876\u2010019\u201001690\u2010w"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0RA04736H"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c04142"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.11.036"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2021.08.033"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.1741"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010016\u20102206\u20104"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12344"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2017\u20100162OC"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2019101085"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1667251"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.119919"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201016544\u20107"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5598291"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.130066"},{"key":"e_1_2_9_28_1","first-page":"571","article-title":"Interstitial cystitis","volume":"23","author":"Powell TO","year":"1955","journal-title":"J Int Coll Surg"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.01305"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061466"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.119961"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1590\/s0100\u2010879x2009005000021"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/phy2.260"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2018.04.013"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464\u2010410X.2007.07111.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.03.022"},{"key":"e_1_2_9_37_1","first-page":"42","article-title":"Urologic chronic pelvic pain syndrome\u2010\u2010looking back and looking forward","volume":"51","author":"Dimitrakov J","year":"2009","journal-title":"Folia Med (Plovdiv)"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2011.01.023"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2021.1898583"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0000000000000963"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.1910"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0b013e31823cd524"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201010\u2010199"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.09.142"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11934\u2010006\u20100051\u20108"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/120525"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.11.007"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.2337\/dbi15\u20100019"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.33590\/emjurol\/10314273"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12657"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902187"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005392\u2010200202000\u201000068"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010020\u201002859\u20102"},{"key":"e_1_2_9_54_1","first-page":"3936","article-title":"In vitro effects of 4\u2010hydroperoxycyclophosphamide on the morphology and function of human peripheral blood mononuclear phagocytic cells (macrophages)","volume":"44","author":"Jason M","year":"1984","journal-title":"Cancer Res"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015230\u2010y"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.20.040180.002301"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/9355692"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26652"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.3758\/bf03193146"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022\u20105347(05)64746\u20105"}],"container-title":["Bioengineering &amp; Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btm2.10346","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/btm2.10346","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btm2.10346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:09:32Z","timestamp":1760047772000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btm2.10346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,13]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/btm2.10346"],"URL":"https:\/\/doi.org\/10.1002\/btm2.10346","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/BTM2.10346\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10346\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10346\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10346\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10346\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10346\/v1\/review1","asserted-by":"object"}]},"ISSN":["2380-6761","2380-6761"],"issn-type":[{"value":"2380-6761","type":"print"},{"value":"2380-6761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,13]]},"assertion":[{"value":"2022-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10346"}}