{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:18:23Z","timestamp":1791317903269,"version":"4.3.1"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["NIH R01 AR074627"],"award-info":[{"award-number":["NIH R01 AR074627"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"publisher","award":["K01 EB031984"],"award-info":[{"award-number":["K01 EB031984"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioengineering &amp;amp; Transla Med"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Injuries caused by surgical incisions or traumatic lacerations compromise the structural and functional integrity of skin. Immediate approximation and robust repair of skin are critical to minimize occurrences of dehiscence and infection that can lead to impaired healing and further complication. Light\u2010activated skin sealing has emerged as an alternative to sutures, staples, and superficial adhesives, which do not integrate with tissues and are prone to scarring and infection. Here, we evaluate both shorter\u2010 and longer\u2010term efficacy of tissue repair response following laser\u2010activated sealing of full\u2010thickness skin incisions in immunocompetent mice and compare them to the efficacy seen with sutures. Laser\u2010activated sealants (LASEs) in which, indocyanine green was embedded within silk fibroin films, were used to form viscous pastes and applied over wound edges. A hand\u2010held, near\u2010infrared laser was applied over the incision, and conversion of the light energy to heat by the LASE facilitated rapid photothermal sealing of the wound in approximately 1\u2009min. Tissue repair with LASEs was evaluated using functional recovery (transepidermal water loss), biomechanical recovery (tensile strength), tissue visualization (ultrasound [US] and photoacoustic imaging [PAI]), and histology, and compared with that seen in sutures. Our studies indicate that LASEs promoted earlier recovery of barrier and mechanical function of healed skin compared to suture\u2010closed incisions. Visualization of sealed skin using US and PAI indicated integration of the LASE with the tissue. Histological analyses of LASE\u2010sealed skin sections showed reduced neutrophil and increased proresolution macrophages on Days 2 and 7 postclosure of incisions, without an increase in scarring or fibrosis. Together, our studies show that simple fabrication and application methods combined with rapid sealing of wound edges with improved histological outcomes make LASE a promising alternative for management of incisional wounds and lacerations.<\/jats:p>","DOI":"10.1002\/btm2.10412","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T04:52:25Z","timestamp":1664340745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Temporal evaluation of efficacy and quality of tissue repair upon laser\u2010activated sealing"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5948-6995","authenticated-orcid":false,"given":"Deepanjan","family":"Ghosh","sequence":"first","affiliation":[{"name":"Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5038-096X","authenticated-orcid":false,"given":"Christopher M.","family":"Salinas","sequence":"additional","affiliation":[{"name":"James C. Wyant College of Optical Sciences University of Arizona  Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubham","family":"Pallod","sequence":"additional","affiliation":[{"name":"Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Roberts","sequence":"additional","affiliation":[{"name":"School of Life Sciences Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inder Raj S.","family":"Makin","sequence":"additional","affiliation":[{"name":"School of Osteopathic Medicine A.T. Still University  Mesa Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4133-474X","authenticated-orcid":false,"given":"Jordan R.","family":"Yaron","sequence":"additional","affiliation":[{"name":"Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University  Tempe Arizona USA"},{"name":"Department of Chemical Engineering, School for Engineering of Matter, Transport, and Energy Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell S.","family":"Witte","sequence":"additional","affiliation":[{"name":"James C. Wyant College of Optical Sciences University of Arizona  Tucson Arizona USA"},{"name":"Department of Medical Imaging University of Arizona  Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaushal","family":"Rege","sequence":"additional","affiliation":[{"name":"Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University  Tempe Arizona USA"},{"name":"Department of Chemical Engineering, School for Engineering of Matter, Transport, and Energy Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02238246"},{"issue":"5","key":"e_1_2_9_3_1","first-page":"645","article-title":"Stapled vs. hand\u2010sewn colorectal anastomosis in complicated colorectal cancer\u2014a retrospective 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