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Our 3D bio\u2010printed larynx was generated using extrusion\u2010based 3D bioprinter with rabbit's chondrocyte\u2010laden gelatin methacryloyl (GelMA)\/glycidyl\u2010methacrylated hyaluronic acid (GMHA) hybrid bioink. We used a polycaprolactone (PCL) outer framework incorporated with pores to achieve the structural strength of printed constructs, as well as to provide a suitable microenvironment to support printed cells. Notably, we established a novel fluidics supply (FS) system that simultaneously supplies basal medium together with a 3D bioprinting process, thereby improving cell survival during the printing process. Our results showed that the FS system enhanced post\u2010printing cell viability, which enabled the generation of a large\u2010scale cell\u2010laden artificial laryngeal framework. Additionally, the incorporation of the PCL outer framework with pores and inner hydrogel provides structural stability and sufficient nutrient\/oxygen transport. An animal study confirmed that the transplanted 3D bio\u2010larynx successfully maintained the airway. With further development, our new strategy holds great potential for fabricating human\u2010scale larynxes with in vivo\u2010like biological functions for laryngectomy patients.<\/jats:p>","DOI":"10.1002\/btm2.10423","type":"journal-article","created":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T10:59:03Z","timestamp":1666263543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Fluidic integrated\n                    <scp>3D<\/scp>\n                    bioprinting system to sustain cell viability towards larynx fabrication"],"prefix":"10.1002","volume":"8","author":[{"given":"Hae Sang","family":"Park","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine Hallym University  Chuncheon Republic of Korea"},{"name":"Nano\u2010Bio Regenerative Medical Institute, School of Medicine Hallym University  Chuncheon Republic of Korea"},{"name":"Institute of New Frontier Research Team Hallym University, Hallym Clinical and Translation Science Institute  Chuncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Seung","family":"Lee","sequence":"additional","affiliation":[{"name":"Nano\u2010Bio Regenerative Medical Institute, School of Medicine Hallym University  Chuncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Beom","family":"Kim","sequence":"additional","affiliation":[{"name":"Intelligent Robot Research Team Electronics and Telecommunications Research Institute  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang\u2010Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Advanced Materials Science and Engineering, College of Engineering Kangwon National University  Chuncheon Republic of 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