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To overcome this, we have developed a screen\u2010printed carbon electrode (SPCE) immunosensor for rapid PoC detection of JEV nonstructural 1 (NS1) antigen (Ag), found circulating in serum of infected individuals using a smartphone based portable \u201cSensit\u201d device. The modification of SPCE surface with JEV NS1 antibody (Ab) was confirmed via observation of globular protein structures via scanning electron microscopy (SEM), increase in electrode surface hydrophilicity via contact angle measurement and decrease in current via differential pulse voltammetry (DPV). The fabrication and testing parameters were optimized based on highest current output obtained using DPV. The SPCE was tested for detection limit of target JEV NS1 Ag ranging from 1\u00a0fM to 1\u00a0\u03bcM, which was determined as 0.45\u2009fM in spiked serum. The disposable immunosensor was also found to be highly specific in detecting JEV NS1 Ag over other flaviviral NS1 Ag. Finally, the modified SPCE was clinically validated by testing 62 clinical JEV samples using both a portable miniaturized electrochemical \u201cSensit\u201d device coupled with a smartphone and a laboratory\u2010based potentiostat. The results were corroborated with gold\u2010standard RT\u2010PCR and showed 96.77% accuracy, 96.15% sensitivity, and 97.22% specificity. Hence, this technique may further be developed into a one\u2010step rapid diagnostic tool for JEV, especially in rural areas.<\/jats:p>","DOI":"10.1002\/btm2.10506","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T03:48:33Z","timestamp":1681357713000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Point\u2010of\u2010care detection of Japanese encephalitis virus biomarker in clinical samples using a portable smartphone\u2010enabled electrochemical \u201cSensit\u201d device"],"prefix":"10.1002","volume":"8","author":[{"given":"Akanksha","family":"Roberts","sequence":"first","affiliation":[{"name":"DBT\u2010National Institute of Animal Biotechnology (NIAB)  Hyderabad Telangana India"},{"name":"DBT\u2010Regional Centre for Biotechnology (RCB)  Faridabad Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himani","family":"Dhanze","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Veterinary Research Institute (IVRI)  Izatnagar Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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