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Soluble CD80 can induce T cell activation and overcome PDL1\u2010mediated immune suppression. In this study, we aimed to construct recombinant\n                    <jats:italic>Lactococcus lactis<\/jats:italic>\n                    for oral delivery of the soluble CD80 (hsCD80) protein or the fusion protein containing the cholera toxin B subunit (CTB) and hsCD80 (CTB\u2010hsCD80) under the control of the nisin\u2010inducible expression system. The recombinant\n                    <jats:italic>L. lactis<\/jats:italic>\n                    expressed and secreted hsCD80 or CTB\u2010hsCD80 fusion proteins after induction by nisin in vitro and in the enteric cavity. Additionally, the CTB\u2010hsCD80 fusion protein showed uptake by intestinal epithelial cells, was cleaved by the furin protease, and was released as free hsCD80 protein into the blood circulation. Orally administered hsCD80 and CTB\u2010hsCD80 containing\n                    <jats:italic>L. lactis<\/jats:italic>\n                    increased the proportion of activated T cells in the spleen and intestinal epithelium, inhibited tumor growth, and prolonged the survival of tumor\u2010bearing mice. The hsCD80\u2010containing L.\n                    <jats:italic>lactis<\/jats:italic>\n                    showed greater therapeutic effects on primary colonic adenoma in APC\n                    <jats:sup>min\/\u2212<\/jats:sup>\n                    mice and completely suppressed tumor growth. Further, recombinant CTB\u2010hsCD80 in\n                    <jats:italic>L. lactis<\/jats:italic>\n                    was more efficient than hsCD80\u2010containing bacteria in inhibiting the growth of xenografted colon cancer and melanoma cells. hsCD80 engineered probiotics may serve as a promising new approach for antitumor immunotherapy, especially for colorectal cancer.\n                  <\/jats:p>","DOI":"10.1002\/btm2.10533","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T01:12:03Z","timestamp":1683162723000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Soluble\n                    <scp>CD80<\/scp>\n                    oral delivery by recombinant Lactococcus suppresses tumor growth by enhancing\n                    <scp>antitumor<\/scp>\n                    immunity"],"prefix":"10.1002","volume":"8","author":[{"given":"Ziqing","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Cell Biology, School of Basic Medicine Southern Medical University  Guangzhou China"},{"name":"Guangzhou Virotech Phamaceutical Co., Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqing","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Basic Medicine Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zerong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Basic Medicine Southern Medical University  Guangzhou China"},{"name":"Department of Urology, Nanfang Hospital Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Basic Medicine Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Basic Medicine Southern Medical University  Guangzhou China"},{"name":"Department of Dermatology, Dermatology Hospital of Southern Medical University Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xufeng","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Basic Medicine Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjie","family":"Wen","sequence":"additional","affiliation":[{"name":"Guangzhou Weisengene Biological Technology Co., Ltd.  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