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However, the greatest challenge in tracheal\u2010replacement therapy is the promotion of tissue regeneration by rapid graft vascularization. Traditional prevascularization methods for tracheal grafts typically utilize omentum or muscle flap wrapping, which requires a second operation; vascularized segment tracheal orthotopic transplantation in one step remains difficult. This study proposes a method to construct a tissue\u2010engineered tracheal graft, which directly forms the microvascular network after orthotopic transplantation in vivo. The focus of this study was the preparation of a hybrid tracheal graft that is non\u2010immunogenic, has good biomechanical properties, supports cell proliferation, and quickly vascularizes. The results showed that vacuum\u2010assisted decellularized trachea\u2010polycaprolactone hybrid scaffold could match most of the above requirements as closely as possible. 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