{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:22:50Z","timestamp":1789770170606,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T00:00:00Z","timestamp":1692403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016341","name":"Korea University Guro Hospital","doi-asserted-by":"publisher","award":["K2311211"],"award-info":[{"award-number":["K2311211"]}],"id":[{"id":"10.13039\/100016341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019R1A6A3A01091920"],"award-info":[{"award-number":["2019R1A6A3A01091920"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1F1A1068910"],"award-info":[{"award-number":["2020R1F1A1068910"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1I1A1A01042679"],"award-info":[{"award-number":["2021R1I1A1A01042679"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2022R1I1A1A01054001"],"award-info":[{"award-number":["2022R1I1A1A01054001"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioengineering &amp;amp; Transla Med"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Encephaloduroarteriosynangiosis (EDAS), an indirect anastomosis procedure, is widely accepted as a primary treatment for moyamoya disease (MMD) to improve collateral blood flow. During surgical intervention, dural fibroblasts (DuF) are thought to produce various proteins that create an angiogenic microenvironment. However, the biophysiological evidence supporting the angiogenic properties of this surgical technique has not been thoroughly elucidated. The purpose of these studies was to determine whether DuF releases pro\u2010angiogenic factors and chemokines and promotes angiogenic properties in human endothelial cells (ECs) under IL\u20101\u03b2\u2010mediated wound conditions, which are expected to occur during the process of neo\u2010vascularization within the dura mater. Furthermore, a microfluidic chemotaxis platform was implemented to investigate the angiogenic activity of ECs in response to a reconstituted dura model. Transcriptome sequencing revealed that IL\u20101\u03b2 stimulation on DuF induced a significant upregulation of various pro\u2010angiogenic genes, including IL\u20106, IL\u20108, CCL\u20102, CCL\u20105, SMOC\u20101, and SCG\u20102 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Moreover, compared to ECs cultured in na\u00efve media or na\u00efve DuF media, those exposed to IL\u20101\u03b2\u2010DuF conditioned media expressed higher mRNA and protein levels of these pro\u2010angiogenic factors (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). ECs co\u2010cultured with IL\u20101\u03b2\u2010DuF also exhibited considerable migration on the microfluidic chemotaxis platform. Furthermore, the chemotactic effects on the ECs were reduced upon neutralization of IL\u20108 or inhibition of NF\u2010\u03baB signaling. Our findings demonstrate that IL\u20101\u03b2\u2010DuFs release factors that activate and enhance the angiogenic properties of ECs. These results suggest a potential interaction between DuF and ECs following EDAS for MMD, and these components could be targeted for the development of therapeutic biomarkers.\n                  <\/jats:p>","DOI":"10.1002\/btm2.10589","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T18:04:46Z","timestamp":1692641086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Role of human dural fibroblasts in the angiogenic responses of human endothelial cells: An in vitro dural model and the application of lab\u2010on\u2010a\u2010chip for\n                    <scp>EDAS<\/scp>"],"prefix":"10.1002","volume":"8","author":[{"given":"Woo\u2010Keun","family":"Kwon","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Korea University Guro Hospital Korea University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Min","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Graduate School of Medicine Korea University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang Hun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Korea University Anam Hospital Korea University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae\u2010Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Graduate School of Medicine Korea University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u2010Gi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Graduate School of Medicine Korea University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4107-2006","authenticated-orcid":false,"given":"Min\u2010Ho","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Graduate School of Medicine Korea University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyuk","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Graduate School of Medicine Korea University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,19]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"1533","article-title":"Pathophysiology of vascular stenosis and remodeling in moyamoya disease","volume":"12","author":"Fox BM","year":"2021","journal-title":"Front Neurol"},{"issue":"1","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.5853\/jos.2015.01627","article-title":"Moyamoya disease: epidemiology, clinical features, and diagnosis","volume":"18","author":"Kim JS","year":"2016","journal-title":"J Stroke"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"e175","DOI":"10.1016\/j.wneu.2017.09.127","article-title":"Intracranial aneurysms in adult moyamoya disease","volume":"109","author":"Kim JH","year":"2018","journal-title":"World Neurosurg"},{"issue":"6","key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"390","DOI":"10.3340\/jkns.2015.57.6.390","article-title":"Epidemiology of moyamoya disease in Korea: based on national health insurance service data","volume":"57","author":"Kim T","year":"2015","journal-title":"J Korean Neurosurg Soc"},{"issue":"3","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/0887-8994(94)90023-X","article-title":"Encephaloduroarteriosynangiosis (EDAS) for the treatment of childhood moyamoya disease","volume":"10","author":"Ross IB","year":"1994","journal-title":"Pediatr Neurol"},{"issue":"1","key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1227\/NEU.0b013e3181fc5ec2","article-title":"Clinical and angiographic outcomes from indirect revascularization surgery for Moyamoya disease in adults and children: a review of 63 procedures","volume":"68","author":"Dusick JR","year":"2011","journal-title":"Neurosurgery"},{"issue":"4","key":"e_1_2_10_8_1","first-page":"220","article-title":"Additional EDAS using the frontal branch of the superficial temporal artery (STA) and the occipital artery for pediatric moyamoya patients in whom EDAS using the parietal branch of STA was insufficient","volume":"13","author":"Tenjin H","year":"1997","journal-title":"Childs Nerv Syst"},{"issue":"1","key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"S116","DOI":"10.1227\/NEU.0000000000000229","article-title":"Advances and surgical considerations in the treatment of moyamoya disease","volume":"74","author":"Arias EJ","year":"2014","journal-title":"Neurosurgery"},{"issue":"6","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.3174\/ajnr.A4675","article-title":"Angiographic structural differentiation between native arteriogenesis and therapeutic synangiosis in intracranial arterial steno\u2010occlusive disease","volume":"37","author":"Ooi YC","year":"2016","journal-title":"AJNR Am J Neuroradiol"},{"issue":"2","key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/s12975-020-00834-9","article-title":"Endothelial progenitor cells induce angiogenesis: a potential mechanism underlying neovascularization in encephaloduroarteriosynangiosis","volume":"12","author":"Wang Q\u2010N","year":"2021","journal-title":"Transl Stroke Res"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.29738","article-title":"Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI","volume":"6","author":"Absinta M","year":"2017","journal-title":"Elife"},{"issue":"6","key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/j.molmed.2018.04.003","article-title":"Advances in meningeal immunity","volume":"24","author":"Rua R","year":"2018","journal-title":"Trends Mol Med"},{"issue":"6","key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.3171\/2010.12.JNS101732","article-title":"The collagenic architecture of human dura mater","volume":"114","author":"Protasoni M","year":"2011","journal-title":"J Neurosurg"},{"issue":"9","key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1038\/nn.4610","article-title":"High\u2010dimensional, single\u2010cell characterization of the brain's immune compartment","volume":"20","author":"Korin B","year":"2017","journal-title":"Nat Neurosci"},{"issue":"2","key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.4161\/intv.22823","article-title":"In vivo dynamics of innate immune sentinels in the CNS","volume":"1","author":"Nayak D","year":"2012","journal-title":"Intravital"},{"issue":"1","key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s41583-021-00525-w","article-title":"Emerging roles for CNS fibroblasts in health, injury and disease","volume":"23","author":"Dorrier CE","year":"2022","journal-title":"Nat Rev Neurosci"},{"issue":"4","key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1016\/j.cell.2020.12.040","article-title":"Functional characterization of the dural sinuses as a neuroimmune interface","volume":"184","author":"Rustenhoven J","year":"2021","journal-title":"Cell"},{"issue":"12","key":"e_1_2_10_19_1","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1038\/s41590-019-0557-0","article-title":"Cytokines in the balance","volume":"20","year":"2019","journal-title":"Nat Immunol"},{"issue":"1","key":"e_1_2_10_20_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/imr.12621","article-title":"Overview of the IL\u20101 family in innate inflammation and acquired immunity","volume":"281","author":"Dinarello CA","year":"2018","journal-title":"Immunol Rev"},{"issue":"2","key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1161\/STROKEAHA.117.018563","article-title":"Surgical management of moyamoya disease","volume":"49","author":"Acker G","year":"2018","journal-title":"Stroke"},{"issue":"5","key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"541","DOI":"10.3171\/2018.5.PEDS18163","article-title":"Indirect revascularization with the dural inversion technique for pediatric moyamoya disease: 20\u2010year experience","volume":"22","author":"Gadgil N","year":"2018","journal-title":"J Neurosurg Pediatr"},{"issue":"5","key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1007\/s00381-004-1118-9","article-title":"Revascularisation surgery for paediatric moyamoya: a review of the literature","volume":"21","author":"Fung LW","year":"2005","journal-title":"Childs Nerv Syst"},{"issue":"2","key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1016\/S0303-8467(97)00033-4","article-title":"Natural history of moyamoya disease: comparison of activity of daily living in surgery and non surgery groups","volume":"99","author":"Choi JU","year":"1997","journal-title":"Clin Neurol Neurosurg"},{"key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.3389\/fimmu.2019.01426","article-title":"IL\u20101 family cytokine regulation of vascular permeability and angiogenesis","volume":"10","author":"Fahey E","year":"2019","journal-title":"Front Immunol"},{"key":"e_1_2_10_26_1","article-title":"Interleukin\u20101\u03b2 enhances cell adhesion in human endothelial cells via microRNA\u20101914\u20135p suppression","volume":"27","author":"Kihara T","year":"2021","journal-title":"Biochem Biophys Rep"},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"3288","DOI":"10.3389\/fimmu.2020.613170","article-title":"Alternative pathways of IL\u20101 activation, and its role in health and disease","volume":"11","author":"Pyrillou K","year":"2020","journal-title":"Front Immunol"},{"key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"703944","DOI":"10.3389\/fncel.2021.703944","article-title":"Living on the edge of the CNS: meninges cell diversity in health and disease","volume":"15","author":"Derk J","year":"2021","journal-title":"Front Cell Neurosci"},{"key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1126\/sciimmunol.aav0492","article-title":"The anatomy and immunology of vasculature in the central nervous system","volume":"4","author":"Mastorakos P","year":"2019","journal-title":"Sci Immunol"},{"issue":"1","key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12974-020-02005-x","article-title":"Cellular infiltration in traumatic brain injury","volume":"17","author":"Alam A","year":"2020","journal-title":"J Neuroinflammation"},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3389\/fncel.2015.00018","article-title":"Interleukin\u20101 and acute brain injury","volume":"9","author":"Murray KN","year":"2015","journal-title":"Front Cell Neurosci"},{"issue":"4","key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nrrheum.2010.4","article-title":"IL\u20101 pathways in inflammation and human diseases","volume":"6","author":"Gabay C","year":"2010","journal-title":"Nat Rev Rheumatol"},{"issue":"105","key":"e_1_2_10_33_1","article-title":"Interleukin\u20101 (IL\u20101) pathway","volume":"3","author":"Weber A","year":"2010","journal-title":"Sci Signal"},{"issue":"10","key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"8508","DOI":"10.1074\/jbc.M208231200","article-title":"Angiogenic effects of interleukin 8 (CXCL8) in human intestinal microvascular endothelial cells are mediated by CXCR2","volume":"278","author":"Heidemann J","year":"2003","journal-title":"J Biol Chem"},{"issue":"6","key":"e_1_2_10_35_1","first-page":"3369","article-title":"IL\u20108 directly enhanced endothelial cell survival, proliferation, and matrix metalloproteinases production and regulated angiogenesis","volume":"170","author":"Li A","year":"2003","journal-title":"J Immunol Res"},{"issue":"7","key":"e_1_2_10_36_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0158839","article-title":"Interleukin\u20106 induces vascular endothelial growth factor\u2010C expression via Src\u2010FAK\u2010STAT3 signaling in lymphatic endothelial cells","volume":"11","author":"Huang Y\u2010H","year":"2016","journal-title":"PloS One"},{"issue":"1","key":"e_1_2_10_37_1","doi-asserted-by":"crossref","first-page":"7689","DOI":"10.1038\/s41598-021-86913-9","article-title":"Interleukin\u20106 as an enhancer of anti\u2010angiogenic therapy for ovarian clear cell carcinoma","volume":"11","author":"Seki T","year":"2021","journal-title":"Sci Rep"},{"issue":"11","key":"e_1_2_10_38_1","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1089\/wound.2014.0594","article-title":"Chemokine regulation of angiogenesis during wound healing","volume":"4","author":"Bodnar RJ","year":"2015","journal-title":"Adv Wound Care (New Rochelle)"},{"issue":"11","key":"e_1_2_10_39_1","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.3390\/ijms17111856","article-title":"The role of CC\u2010chemokines in the regulation of angiogenesis","volume":"17","author":"Ridiandries A","year":"2016","journal-title":"Int J Mol Sci"},{"issue":"2","key":"e_1_2_10_40_1","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1093\/cvr\/cvv098","article-title":"Role of secreted modular calcium\u2010binding protein 1 (SMOC1) in transforming growth factor \u03b2 signalling and angiogenesis","volume":"106","author":"Awwad K","year":"2015","journal-title":"Cardiovasc Res"},{"issue":"8","key":"e_1_2_10_41_1","doi-asserted-by":"crossref","first-page":"3779","DOI":"10.7150\/thno.44115","article-title":"Fasting before or after wound injury accelerates wound healing through the activation of pro\u2010angiogenic SMOC1 and SCG2","volume":"10","author":"Luo MJ","year":"2020","journal-title":"Theranostics"}],"container-title":["Bioengineering &amp; Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btm2.10589","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:42:37Z","timestamp":1760053357000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btm2.10589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,19]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1002\/btm2.10589"],"URL":"https:\/\/doi.org\/10.1002\/btm2.10589","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/BTM2.10589\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10589\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10589\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10589\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BTM2.10589\/v1\/decision1","asserted-by":"object"}]},"ISSN":["2380-6761","2380-6761"],"issn-type":[{"value":"2380-6761","type":"print"},{"value":"2380-6761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,19]]},"assertion":[{"value":"2023-05-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10589"}}