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It prevents brain damage induced by acute ischemia stroke (AIS). Pyroptosis, executed by the pore\u2010forming protein gasdermin D (GSDMD), is a type of regulated cell death triggered by proinflammatory signals. It contributes to the pathogenesis of ischemic brain injury. However, the effects of RIPostC on pyroptosis following AIS remain largely unknown. In our study, linear correlation analysis confirmed that serum GSDMD levels in AIS patients upon admission were positively correlated with NIHSS scores. RIPostC treatment significantly reduced GSDMD level compared with patients without RIPostC at 3\u2009days post\u2010treatment. Besides, middle cerebral artery occlusion (MCAO) surgery was performed on C57BL\/6 male mice and RIPostC was induced immediately after MCAO. We found that RIPostC suppressed the activation of NLRP3 inflammasome to reduce the maturation of GSDMD, leading to decreased pyroptosis in microglia after AIS. Hepatocyte growth factor (HGF) was identified using the high throughput screening. Importantly, HGF siRNA, exogenous HGF, and ISG15 siRNA were used to reveal that HGF\/ISG15 is a possible mechanism of RIPostC regulation in vivo and in vitro.<\/jats:p>","DOI":"10.1002\/btm2.10590","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T18:03:48Z","timestamp":1692641028000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Remote limb ischemic postconditioning inhibits microglia pyroptosis by modulating\n                    <scp>HGF<\/scp>\n                    after acute ischemia stroke"],"prefix":"10.1002","volume":"8","author":[{"given":"Lu","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Neurology The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuohui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of 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