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Current countermeasures require near immediate administration following OP exposure and are reported to have controversial efficacies. Acetylcholinesterase (AChE) fused to the human immunoglobulin 1 (IgG1) Fc domain (AChE\u2010Fc) is a potential bioscavenger for OP toxicants, but a reproducible AChE\u2010Fc biomanufacturing strategy remains elusive. This report is the first to establish a comprehensive laboratory\u2010scale bioprocessing strategy that can reproducibly produce AChE\u2010Fc and AChE(W86A)\u2010Fc which is a mutated AChE protein with reduced enzymatic activity. Characterization studies revealed that AChE\u2010Fc and AChE(W86A)\u2010Fc are\n                    <jats:italic>N<\/jats:italic>\n                    \u2010glycosylated dimeric fusion glycoproteins but only AChE\u2010Fc had the capability to bind to paraoxon (a model OP). This AChE\u2010Fc fusion glycoprotein bioprocessing strategy can be leveraged during industrial biomanufacturing development, while the research\u2010grade AChE\u2010Fc proteins can be used to determine the potential clinical relevance of the countermeasure against OP toxicants.\n                  <\/jats:p>","DOI":"10.1002\/btm2.10666","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T02:39:42Z","timestamp":1714099182000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Model\n                    <scp>acetylcholinesterase\u2010Fc<\/scp>\n                    fusion glycoprotein biotechnology system for the manufacture of an organophosphorus toxicant bioscavenging countermeasure"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8592-6184","authenticated-orcid":false,"given":"Thomas 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