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Ideally, potent and persistent vaccine effects would be induced with a single vaccine dose. Here, we designed a virus\u2010like particle (VLP)\u2010based vaccine presenting multiple copies of the influenza hemagglutinin (HA) from A\/Puerto Rico\/8\/1934 (PR8HA\u2010VLP) and examined its immunogenicity and protective efficacy in ferrets. Serum\u2010neutralizing antibodies were effectively induced against the homologous virus at 3\u2010week post\u2010vaccination with a single dose of PR8HA\u2010VLP with or without adjuvants. When the single\u2010immunized ferrets were challenged with the homologous virus, virus replication in the nasal mucosa was significantly reduced. Long\u2010term monitoring of serum titers revealed that after adjuvanted vaccination with PR8HA\u2010VLP, neutralizing antibodies were retained at similar levels 20\u2010 to 183\u2010week post\u2010vaccination, although a 4\u2010 to 8\u2010fold titer decline was observed from 3\u2010 to 20\u2010week post\u2010vaccination. Boost immunization at 183\u2009weeks after the first immunization elicited higher neutralizing antibody titers than those at 3\u2009weeks after the initial immunization in most of the animals. These results confirm that nanoparticle\u2010based vaccines are a promising approach to effectively elicit durable multiyear neutralizing antibody responses against influenza viruses.<\/jats:p>","DOI":"10.1002\/btm2.10689","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T08:36:08Z","timestamp":1717403768000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Single immunization with an influenza <scp>hemagglutinin<\/scp> nanoparticle\u2010based vaccine elicits durable protective immunity"],"prefix":"10.1002","volume":"9","author":[{"given":"Shiho","family":"Chiba","sequence":"first","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine Influenza Research Institute, University of Wisconsin\u2010Madison  Madison Wisconsin USA"},{"name":"Pandemic Preparedness, Infection and Advanced Research Center (UTOPIA) The University of Tokyo  Tokyo 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Influenza Research Institute, University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4999-4697","authenticated-orcid":false,"given":"Asim","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine Influenza Research Institute, University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Hatta","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine Influenza Research Institute, University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Kawaoka","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine Influenza Research 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