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However, the continuous extrusion forming with the rigid stationary shaft of the peristaltic pumps can damage cells, leading to metabolic disorders and eventual dysfunction of transplanted organs. Here, we developed a novel biomimetic blood\u2010gas system (BBGs) for preventing cell damage. This system mimics the cardiac cycle and features an adjustable inspiratory\u2010to\u2010expiratory (IE) ratio to mitigate acidosis caused by continuous oxygen inhalation. In our study, adipose stem cells (ADSCs) were cultured within the circulatory system for 10\u2009min, 2, and 4\u2009h. Compared to the peristaltic pump, the BBGs significantly reduced cell apoptosis and morphological injury while enhancing cell proliferation and adhesion. Additionally, when the supernatant from ADSCs was introduced to LPS\u2010induced macrophages for 24\u2009h, the BBGs group demonstrated a more pronounced anti\u2010inflammatory effect, characterized by reduced M1 macrophage expression. Besides, with isolated rat livers from donation after circulatory death (DCD) perfusion with ADSCs for 6\u2009h by the BBGs, we detected fewer apoptotic cells and a reduced inflammatory response, evidenced by down\u2010regulated TNF\u2010\u03b1 expression. The development of BBGs demonstrates the feasibility of recreating physiological liquid\u2013gas circulation in vitro, offering an alternative platform for isolated organ perfusion, especially for applications involving cell therapy.<\/jats:p>","DOI":"10.1002\/btm2.10724","type":"journal-article","created":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T05:39:46Z","timestamp":1727069986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel cytoprotective organ perfusion platform for reconstructing homeostasis of <scp>DCD<\/scp> liver while alleviating <scp>IRI<\/scp> injury"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4865-4092","authenticated-orcid":false,"given":"Tingting","family":"Lan","sequence":"first","affiliation":[{"name":"Research Institute of Transplant Medicine, Tianjin First Central Hospital, School of Medicine, Nankai University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingxing","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology College of Life Science, Nankai University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Ming","sequence":"additional","affiliation":[{"name":"Research Institute of Transplant Medicine, Tianjin First Central Hospital, School of Medicine, Nankai University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Deng","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, Chinese Academy of Medical Sciences &amp; Peking Union 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