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Fecal calprotectin is an established biomarker for ulcerative colitis (UC), one of the main IBD diseases, which provides indications of the presence and severity of inflammation in the digestive tract. Enzyme\u2010Linked Immunosorbent Assay (ELISA) as a gold standard approach for fecal calprotectin detection is time\u2010consuming and impractical in point\u2010of\u2010care settings. Moreover, obtaining fecal samples from patients is challenging and inhibits longitudinal monitoring. To address these specific problems, we have developed a novel approach for detecting calprotectin which leverages clustered regularly interspaced short palindromic repeats (CRISPR)\/Cas technology. We successfully developed a portable tube\u2010based CRISPR\/Cas assay for point\u2010of\u2010care testing of calprotectin. This assay showed a detection range from 1 to 10,000\u2009ng\/ml (over 4 log units), using both fluorescent and colorimetric analytical techniques. The established assay was further validated through measurements in mucosal samples obtained in an anesthetised preclinical rodent model of UC, with 2\u20133 times higher calprotectin concentration detected in UC rat samples compared to that of healthy control animals. This point\u2010of\u2010care test may provide a rapid, precise, and user\u2010friendly approach for the diagnosis and monitoring of IBD through mucosal sample testing.<\/jats:p>","DOI":"10.1002\/btm2.10725","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T11:15:09Z","timestamp":1727262909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A CRISPR mediated point\u2010of\u2010care assay for the detection of mucosal calprotectin in an animal model of ulcerative colitis"],"prefix":"10.1002","volume":"10","author":[{"given":"Selena","family":"Chia","sequence":"first","affiliation":[{"name":"Graduate School of Biomedical Engineering UNSW Sydney  Sydney New South Wales Australia"},{"name":"Tyree Foundation Institute of Health Engineering (iHealthE) UNSW Sydney  New South Wales 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