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The purpose of this study is to verify the feasibility of CD47 as an optical molecular imaging (OMI) target for human EC and to achieve precise localization and identification in hysteroscopic surgery. The results demonstrated that CD47 was overexpressed in EC through bioinformatics, immunohistochemistry, and qRT\u2010PCR. In EC cell lines, CD47\u2010targeted near\u2010infrared photoimmunotherapy (NIR\u2010PIT) induced cytotoxicity in a light dose\u2010dependent manner. Laser confocal microscopy revealed that CD47 intervention significantly increased the phagocytic effect of macrophages on EC cells. In the mice model of partial tumor resection mediated by CD47\u2010targeted OMI, compared to group A (immune therapy alone), group C (NIR\u2010PIT treatment) mice showed a reduced tumor recurrence rate after NIR\u2010PIT intervention. However, the difference did not reach statistical significance. We then evaluated the effect of CD47\u2010targeted NIR\u2010PIT maintenance therapy on tumor recurrence in mice. The results indicated that, compared to untreated animals, the tumor growth rate was slower in the NIR\u2010PIT group using CD47\u2010Alexa Fluor 790 (CD47\u2010AF790), allowing for more sustained tumor control. The freshly isolated whole uterus specimens from EC patients were co\u2010incubated with CD47\u2010AF790, and a significantly enhanced contrast of NIR visible images of tumor tissue was observed, demonstrating high sensitivity and specificity (tumor\u2010to\u2010background ratio &gt;5.05). Finally, under fluorescence microscopy, specific fluorescent signals are observed on tumor cells. In conclusion, accurate localization and excision of EC can be accomplished by employing CD47 optical molecular contrast agents with OMI technology. This method shows potential as a viable and promising approach for the precise diagnosis of EC.<\/jats:p>","DOI":"10.1002\/btm2.10754","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T08:09:43Z","timestamp":1738656583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multimodal near\u2010infrared molecular imaging of ex vivo endometrial carcinoma via <scp>CD47<\/scp>\u2010based targeted tracer"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7935-315X","authenticated-orcid":false,"given":"Jing","family":"Lei","sequence":"first","affiliation":[{"name":"Department of Gynecology First Hospital of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dianfeng","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Coloproctology First Hospital of Shanxi Medical 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