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This study investigates the therapeutic potential of engineering biomimetic nanovesicles to deliver phosphatidylethanolamine\u2010binding protein 1 (PEBP1) mRNA for inhibiting ferroptosis in vascular smooth muscle cells (VSMCs) and preventing AAA progression. Differential gene expression analysis of the AAA transcriptomic dataset GSE57691 identified 243 differentially expressed genes (DEGs), intersecting with 12 ferroptosis\u2010related genes. Single\u2010cell analysis of dataset GSE237230 highlighted PEBP1 as a key gene in VSMCs. Overexpression of PEBP1 in VSMCs enhanced proliferation, reduced reactive oxygen species (ROS) and iron levels, and inhibited apoptosis and ferroptosis via the NRF2\/GPX4 axis. The engineered biomimetic nanovesicles demonstrated significant uptake by VSMCs and effective delivery of PEBP1 mRNA. In vivo studies confirmed that these nanovesicles substantially inhibited AAA progression in mice. This study presents a novel bioengineering approach for AAA treatment by targeting ferroptosis through PEBP1 mRNA delivery, offering a promising molecular strategy for the prevention and management of AAA.<\/jats:p>","DOI":"10.1002\/btm2.70025","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T08:05:37Z","timestamp":1753085137000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Engineering biomimetic nanovesicles for <scp>PEBP1 mRNA<\/scp> delivery to inhibit ferroptosis in abdominal aortic aneurysm"],"prefix":"10.1002","volume":"10","author":[{"given":"Lulu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Wenzhou Medical University  Wenzhou PR 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