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Our work investigated a novel combination of non\u2010immunogenic polyelectrolytes, tetrahydropyran triazole phenyl\u2010alginate (TZ\u2010AL) and quaternized phosphocholine\u2010chitosan (PC\u2010QCH), for layer\u2010by\u2010layer self\u2010assembly onto the surface of mouse islets. Building on previous work validating coating characteristics and biocompatibility using cell\u2010derived spheroids, we assessed the immunoprotective properties of the polyelectrolyte coating. This was done through in vitro co\u2010culture of the polyelectrolytes with mouse\u2010derived splenocytes enriched for antigen\u2010presenting cells (APCs) and syngeneic transplantation of coated mouse islets into STZ\u2010induced diabetic mice. Results indicated that the polyelectrolytes may downregulate APC activation and maturation in vitro. In addition, coated islets successfully restored normoglycemia in syngeneic transplants, as demonstrated by blood glucose measurements, intraperitoneal glucose tolerance tests, and graft immunostaining. These results suggest that the polyelectrolyte coating may modulate APC activation and that coated islets exhibit therapeutic efficacy for glycemic control in T1D.<\/jats:p>","DOI":"10.1002\/btm2.70039","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T05:42:01Z","timestamp":1769060521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of a functionalized chitosan and alginate multilayer conformal nanocoating toward improving islet survival in syngeneic mouse islet transplantation"],"prefix":"10.1002","volume":"11","author":[{"given":"Michael Yilma","family":"Yitayew","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering McGill University  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Bay","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology 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