{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T00:45:07Z","timestamp":1792889107659,"version":"4.5.0"},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioengineering &amp;amp; Transla Med"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Osteoarthritis (OA) is a prevalent chronic degenerative joint disease with no known treatment for reversing its progression. However, recent studies have shown promising results for nano\u2010sized extracellular vesicles derived from preconditioned synovial mesenchymal stem cells (SMSCs) in treating various diseases, including OA. Glucocorticoids (GCs) possess potent anti\u2010inflammatory properties, but their long\u2010term use is limited due to potential adverse reactions. Building on previous research, this study aimed to investigate the therapeutic potential of extracellular vesicles secreted from GCs\u2010pretreated SMSCs (GCs\u2010EVs) in exerting antiarthritic effects. The results demonstrated that GCs\u2010EVs effectively inhibited cartilage degeneration and osteophyte formation in the OA mouse model by suppressing the release of inflammatory cytokines from synovial macrophages. This effect was attributed to the high expression of miR\u2010212\u20105p in GCs\u2010EVs, which specifically inhibited the expression of methyltransferase\u2010like 3 (Mettl3). Consequently, the mRNA N6\u2010methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) level of nod\u2010like receptor pyrin domain 3 inflammasome (NLRP3) in macrophages was reduced, leading to decreased NLRP3 inflammasome activity and increased antiarthritic effects. Furthermore, in the co\u2010culture system, GCs\u2010EVs enhanced chondrocyte proliferation and migration while inhibiting chondrocyte apoptosis by suppressing the secretion of inflammatory factors by macrophages.\n                  <\/jats:p>","DOI":"10.1002\/btm2.70042","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:29:09Z","timestamp":1751416149000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Extracellular vesicles from glucocorticoids\u2010preconditioned synovial mesenchymal stem cells exert antiarthritic effects by downregulating the\n                    <scp>mRNA m6A<\/scp>\n                    modification of\n                    <scp>NLRP3<\/scp>\n                    in macrophages through\n                    <scp>miR<\/scp>\n                    \u2010212\u20105p"],"prefix":"10.1002","volume":"11","author":[{"given":"Xiaolong","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Joint Surgery, Xi'an Honghui Hospital Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Practice, Xi'an Honghui Hospital Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouye","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery, Xi'an Honghui Hospital Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8376-8204","authenticated-orcid":false,"given":"Zhi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery, Xi'an Honghui Hospital Xi'an Jiaotong University  Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-204599"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60802-3"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00111"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/284873"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.5312\/wjo.v6.i1.95"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.03.011"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12608"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15905"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.007"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.178"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.191"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S254879"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-021-00906-4"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-014-0432-1"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215157"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.02.022"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0002"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200108)44:8<1928::AID-ART331>3.0.CO;2-P"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.17133"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000029"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-29"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021014-071641"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7BM00479F"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.11.008"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.04.010"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00377"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.03.022"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-020-09559-9"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-1004-0"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0212664"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13492"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050541"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2022.4852"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21984"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2005.038638"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2008.100008"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2008.092163"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.05.031"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.7326\/M20-1594"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09839-x"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2016.1208655"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2016.09.002"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1596481"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-022-00947-8"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20182091"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2025070118"},{"key":"e_1_2_13_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-03005-9"},{"key":"e_1_2_13_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-021-00756-x"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.667644"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v2i0.20677"},{"key":"e_1_2_13_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.502"},{"key":"e_1_2_13_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-319"},{"key":"e_1_2_13_54_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b12-00607"},{"key":"e_1_2_13_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046874"},{"key":"e_1_2_13_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks658"},{"key":"e_1_2_13_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-021-01236-1"},{"key":"e_1_2_13_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02317-6"},{"key":"e_1_2_13_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3462"},{"key":"e_1_2_13_60_1","first-page":"648","article-title":"The prevalence of symptomatic knee osteoarthritis in China: results from the China health and retirement longitudinal study, arthritis","volume":"68","author":"Tang X","year":"2016","journal-title":"Rheumatology"},{"key":"e_1_2_13_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2016.44"},{"key":"e_1_2_13_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26018"},{"key":"e_1_2_13_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.04.031"},{"key":"e_1_2_13_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.816209"},{"key":"e_1_2_13_65_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02507-2"},{"key":"e_1_2_13_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-1726-7"},{"key":"e_1_2_13_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12769"}],"container-title":["Bioengineering &amp; Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btm2.70042","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/btm2.70042","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btm2.70042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:15:46Z","timestamp":1789485346000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btm2.70042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":66,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/btm2.70042"],"URL":"https:\/\/doi.org\/10.1002\/btm2.70042","archive":["Portico"],"relation":{},"ISSN":["2380-6761","2380-6761"],"issn-type":[{"value":"2380-6761","type":"print"},{"value":"2380-6761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,1]]},"assertion":[{"value":"2025-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70042"}}