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Many studies have explored different materials and active cues to guide neural regeneration, with some success. However, none have demonstrated a comparable or better functional recovery than the clinical standard autograft. Autografts are often insufficient for reconstruction of an injury to long nerves such as the sciatic or brachial plexus. Synthetic nerve guidance conduits (NGCs) have been investigated for these injuries to guide axonal regeneration and lead to functional recovery. We have designed a biologics\u2010free hydrogel\u2010based multi\u2010channel conduit with defined microscale features to guide axonal outgrowth. To investigate extraneural vascular infiltration and its effects on functional recovery, we also designed a multi\u2010microchannel conduit with defined regularly spaced micropores, orthogonal to the axon guidance channels. Using our custom\u2010built Rapid Projection, Image\u2010guided, Dynamic (RaPID) bioprinting system, we were able to fabricate each hydrogel conduit within minutes from a milliliter\u2010volume prepolymer vat. With our state\u2010of\u2010the\u2010art printing platform, we have achieved NGCs with a consistent channel wall width of 10\u2009\u03bcm. We implanted the NGCs for 17\u2009weeks in a murine sciatic nerve transection injury model. We assessed the functional recovery by dynamic gait analysis throughout the recovery period and by compound muscle action potential (CMAP) electrophysiology before NGC harvesting. Both the non\u2010porous and micro\u2010porous conduit groups led to functional nerve regeneration on par with the autograft group. Further, both conduit groups resulted in restoration of bulk motor function to pre\u2010injury performance.<\/jats:p>","DOI":"10.1002\/btm2.70057","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T10:15:07Z","timestamp":1754302507000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>3D<\/scp>\n                    printed nerve guidance conduit for biologics\u2010free nerve regeneration and vascular integration"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9028-2632","authenticated-orcid":false,"given":"Jacob","family":"Schimelman","sequence":"first","affiliation":[{"name":"Aiiso Yufeng Li Family Department of Chemical and Nano Engineering University of California San Diego  La Jolla California 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