{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:55:41Z","timestamp":1791197741647,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioengineering &amp;amp; Transla Med"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Breast cancer remains the leading cause of cancer\u2010related mortality among women worldwide. Tumor biomechanics are not merely a symptom: they represent a functional signature with translational relevance in diagnostic, prognostic, and therapeutic resistance. Despite this, few experimental models are engineered to systematically investigate these physical properties across biological systems. Here, this study presents a multimodal biomechanical platform combining engineered 3D breast cancer spheroids with\n                    <jats:italic>ex vivo<\/jats:italic>\n                    tissue analysis to profile and compare viscoelastic behavior of healthy and tumoral environments. Rheometry and compression testing revealed a consistent mechanical shift in tumor\u2010derived samples marked by increased stiffness and force\u2010dependent nonlinear behavior, mirroring the ECM remodeling typical of aggressive phenotypes. This increased rigidity may adversely affect chemotherapy effectiveness by hindering drug delivery and altering cellular mechanotransduction. These biomechanical fingerprints enable quantitative discrimination between healthy and cancerous tissues and can serve as a surrogate marker of malignancy. By supporting the development of mechanics\u2010informed diagnostic tools, our platform offers a reproducible, clinically relevant framework to integrate biomechanical screening into translational breast cancer pipelines.\n                  <\/jats:p>","DOI":"10.1002\/btm2.70148","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T04:46:17Z","timestamp":1778301977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanical fingerprints in breast cancer research: A multimodal experimental approach"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5855-4490","authenticated-orcid":false,"given":"Federica","family":"Banche\u2010Niclot","sequence":"first","affiliation":[{"name":"Center for Musculoskeletal Regeneration Houston Methodist Academic Institute  Houston Texas USA"},{"name":"Orthopedics and Sports Medicine Houston Methodist Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1443-4160","authenticated-orcid":false,"given":"Rosalia","family":"Ferraro","sequence":"additional","affiliation":[{"name":"Department of Chemical, Material and Industrial Production Engineering University of Naples \u201cFederico II\u201d  Naples Italy"},{"name":"CEINGE Advanced Biotechnologies Franco Salvatore  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9233-3552","authenticated-orcid":false,"given":"Valerio","family":"Di Palo","sequence":"additional","affiliation":[{"name":"Department of Chemical, Material and Industrial Production Engineering University of Naples \u201cFederico II\u201d  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"de Paolis","sequence":"additional","affiliation":[{"name":"Department of Chemical, Material and Industrial Production Engineering University of Naples \u201cFederico II\u201d  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4959-1169","authenticated-orcid":false,"given":"Francesca","family":"Taraballi","sequence":"additional","affiliation":[{"name":"Center for Musculoskeletal Regeneration Houston Methodist Academic Institute  Houston Texas USA"},{"name":"Orthopedics and Sports Medicine Houston Methodist Hospital  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4400-0059","authenticated-orcid":false,"given":"Sergio","family":"Caserta","sequence":"additional","affiliation":[{"name":"Department of Chemical, Material and Industrial Production Engineering University of Naples \u201cFederico II\u201d  Naples Italy"},{"name":"CEINGE Advanced Biotechnologies Franco Salvatore  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.breast.2022.08.010"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.09.024"},{"issue":"4","key":"e_1_2_11_4_1","first-page":"269","article-title":"Mechanical properties of human tumour tissues and their implications for cancer development. Nature reviews","volume":"6","author":"Massey A","year":"2024","journal-title":"Physics"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201701039"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40164-024-00591-7"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1273154"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.785138"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4TB00328D"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10911-024-09567-z"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-207-6_11"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06887"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050498"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddtec.2017.03.002"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2005.11.182"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JMSM.0000021108.74004.7e"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40778-024-00239-6"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00721-2"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202202609"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a041661"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.13800"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.05.072"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sna.2017.08.033"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn500553z"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177561"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188346"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/admt.202301890"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2024.101066"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-44738-4_15"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2011.583233"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/52\/5\/003"},{"key":"e_1_2_11_32_1","article-title":"Biomechanical characterization of human soft tissues using indentation and tensile testing","volume":"118","author":"Griffin M","year":"2016","journal-title":"J vis Exp"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2451290"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-016-0139-7"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.08.006"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2024.112016"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.06.018"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-64162-6"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep19103"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2023.100821"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202201842"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-4521"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1088\/2053-1591\/ab4f42"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202000517"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3SM00077J"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2021.04.002"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16234043"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2026.102888"},{"key":"e_1_2_11_49_1","doi-asserted-by":"crossref","unstructured":"Banche\u2010NiclotF FerraroR PaloVD et al.Mechanical fingerprints in breast cancer research: a multimodal experimental approach. bioRxiv. 2025:2025.10.03.680332.","DOI":"10.1101\/2025.10.03.680332"}],"container-title":["Bioengineering &amp; 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