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However, safe and efficient methods for delivering gene editors into primary human cells and tissues remain a central challenge. In this study, we employed a PepFect14 (PF14) analog, PF14\u2010K, to deliver high\u2010fidelity Cas9\u2010ribonucleoproteins and non\u2010viral repair templates into primary human skin cells to mediate gene editing and repair targeting genes underlying the group of genetic skin blistering disorders epidermolysis bullosa (EB). Peptide\u2010RNP nanoparticles enabled consistent gene editing of &gt;70% in primary wild type fibroblasts and &gt;50% in primary wild type keratinocytes. In more difficult\u2010to\u2010transfect primary EB skin cells, this strategy facilitated up to 68% exon deletion\u2010mediated reframing targeting\n                    <jats:italic>COL7A1<\/jats:italic>\n                    and 37% precise homology\u2010directed repair of a prevalent\n                    <jats:italic>LAMB3<\/jats:italic>\n                    mutation. Compared to electroporation, the gold standard for ex vivo delivery, PF14\u2010K enabled similar total yields of edited cells. Deliverable PF14\u2010K nanoparticles are highly cost\u2010effective, as they can be formed on the benchtop through a simple mix\u2010and\u2010incubate approach, with future potential to deliver base and prime editors.\n                  <\/jats:p>","DOI":"10.1002\/btm2.70172","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T07:34:25Z","timestamp":1787902465000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A\n                    <scp>PepFect14<\/scp>\n                    analog improves non\u2010viral\n                    <scp>CRISPR<\/scp>\n                    delivery in primary human cells to facilitate genome editing and repair"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9481-4149","authenticated-orcid":false,"given":"Alex","family":"du Rand","sequence":"first","affiliation":[{"name":"School of Biological Sciences The University of Auckland  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