{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:06:34Z","timestamp":1791227194042,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T00:00:00Z","timestamp":1366243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p>\n                    Butanol is considered as a superior biofuel, which is conventionally produced by clostridial acetone\u2010butanol\u2010ethanol (ABE) fermentation. Among ABE, only butanol and ethanol can be used as fuel alternatives. Coproduction of acetone thus causes lower yield of fuel alcohols. Thus, this study aimed at developing an improved\n                    <jats:italic>Clostridium acetobutylicum<\/jats:italic>\n                    strain possessing enhanced fuel alcohol production capability. For this, we previously developed a hyper ABE producing BKM19 strain was further engineered to convert acetone into isopropanol. The BKM19 strain was transformed with the plasmid pIPA100 containing the\n                    <jats:italic>sadh<\/jats:italic>\n                    (primary\/secondary alcohol dehydrogenase) and\n                    <jats:italic>hydG<\/jats:italic>\n                    (putative electron transfer protein) genes from the\n                    <jats:italic>Clostridium beijerinckii<\/jats:italic>\n                    NRRL B593 cloned under the control of the thiolase promoter. The resulting BKM19 (pIPA100) strain produced 27.9 g\/l isopropanol\u2010butanol\u2010ethanol (IBE) as a fuel alcohols with negligible amount of acetone (0.4 g\/l) from 97.8 g\/l glucose in lab\u2010scale (2 l) batch fermentation. Thus, this metabolically engineered strain was able to produce 99% of total solvent produced as fuel alcohols. The scalability and stability of BKM19 (pIPA100) were evaluated at 200 l pilot\u2010scale fermentation, which showed that the fuel alcohol yield could be improved to 0.37 g\/g as compared to 0.29 g\/g obtained at lab\u2010scale fermentation, while attaining a similar titer. To the best of our knowledge, this is the highest titer of IBE achieved and the first report on the large scale fermentation of\n                    <jats:italic>C. acetobutylicum<\/jats:italic>\n                    for IBE production. \u00a9 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1083\u20131088, 2013\n                  <\/jats:p>","DOI":"10.1002\/btpr.1733","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T11:42:04Z","timestamp":1366285324000},"page":"1083-1088","source":"Crossref","is-referenced-by-count":81,"title":["Metabolic engineering of\n                    <i>Clostridium acetobutylicum<\/i>\n                    for the enhanced production of isopropanol\u2010butanol\u2010ethanol fuel mixture"],"prefix":"10.1002","volume":"29","author":[{"given":"Yu\u2010Sin","family":"Jang","sequence":"first","affiliation":[{"name":"Dept. of Chemical and Biomolecular Engineering (BK21 Program) Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, KAIST Daejeon 305-701 Republic of Korea"},{"name":"BioFuelChem Daejeon 305-732 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alok","family":"Malaviya","sequence":"additional","affiliation":[{"name":"Dept. of Chemical and Biomolecular Engineering (BK21 Program) Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, and Bioinformatics Research Center, KAIST Daejeon 305-701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joungmin","family":"Lee","sequence":"additional","affiliation":[{"name":"Dept. of Chemical and Biomolecular Engineering (BK21 Program) Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, and Bioinformatics Research Center, KAIST Daejeon 305-701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Ae","family":"Im","sequence":"additional","affiliation":[{"name":"Dept. of Chemical and Biomolecular Engineering (BK21 Program) Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, and Bioinformatics Research Center, KAIST Daejeon 305-701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Yup","family":"Lee","sequence":"additional","affiliation":[{"name":"Dept. of Chemical and Biomolecular Engineering (BK21 Program) Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, and Bioinformatics Research Center, KAIST Daejeon 305-701 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Lee","sequence":"additional","affiliation":[{"name":"GS Caltex R&amp;D Center Daejeon 305-380 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moon\u2010Ho","family":"Eom","sequence":"additional","affiliation":[{"name":"GS Caltex R&amp;D Center Daejeon 305-380 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung\u2010Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"GS Caltex R&amp;D Center Daejeon 305-380 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Young","family":"Seung","sequence":"additional","affiliation":[{"name":"GS Caltex R&amp;D Center Daejeon 305-380 Republic of 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