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Cholera toxin B subunit (CTB) has been widely used to facilitate antigen delivery by serving as an effective mucosal carrier molecule for the induction of oral tolerance. We investigate here the application of CTB as a transmucosal carrier in enhancing its fusion protein\u2010LKs effect to protect rats against thrombosis. Thus, in this study, CTB\u2010LK fusion gene separated by a furin cleavage site was expressed in seeds of <jats:italic>Helianthus annuus<\/jats:italic> L. The activity of recombinant protein in seeds of transgenic sunflower was confirmed by Western blot analysis, fibrin plate assays and G<jats:sub>M1<\/jats:sub>\u2010ganglioside ELISA. The thrombosis model of rats and mice revealed that the oral administration of peeled seeds of sunflower expressing CTB\u2010LK had a more significant anti\u2010thrombotic effect on animals compared with that administration of peeled seeds of sunflower expressing LK. It is possible to conclude that CTB can successfully enhance its fusion protein to be absorbed in rats or mice thrombosis model. The use of CTB as a transmucosal carrier in the delivery of transgenic plant\u2010derived oral therapeutic proteins was supported. In addition, for the purpose of that recombinant CTB\u2010LK was designed for oral administration, thus the expression of CTB\u2010LK in edible sunflower seeds eliminated the need for downstream processing of proteins. \u00a9 2014 American Institute of Chemical Engineers <jats:italic>Biotechnol. Prog<\/jats:italic>., 30:1029\u20131039, 2014<\/jats:p>","DOI":"10.1002\/btpr.1963","type":"journal-article","created":{"date-parts":[[2014,7,30]],"date-time":"2014-07-30T18:28:07Z","timestamp":1406744887000},"page":"1029-1039","source":"Crossref","is-referenced-by-count":7,"title":["Expression of cholera toxin B subunit\u2013lumbrokinase in edible sunflower seeds\u2013the use of transmucosal carrier to enhance its fusion protein's effect on protection of rats and mice against thrombosis"],"prefix":"10.1002","volume":"30","author":[{"given":"Chunfeng","family":"Guan","sequence":"first","affiliation":[{"name":"School of Environmental Science and Engineering Tianjin University Tianjin 300072 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Ji","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering Tianjin University Tianjin 300072 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering Tianjin University Tianjin 300072 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering Tianjin University Tianjin 300072 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozhou","family":"Li","sequence":"additional","affiliation":[{"name":"Dept. of Medical Genetics Tianjin Medical University General Hospital Tianjin 300072 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenzhu","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering and Technology Tianjin University Tianjin 300072 People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,8,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/294258"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/03670244.1997.9991517"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(01)00140-4"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053110"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.05.003"},{"volume-title":"Earthworms: sources of antimicrobial and anticancer molecules. 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