{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T21:19:54Z","timestamp":1784755194816,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,7,23]],"date-time":"2009-07-23T00:00:00Z","timestamp":1248307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microorganisms can complex and sequester heavy metals, rendering them promising living factories for nanoparticle production. Glutathione (GSH) is pivotal in cadmium sulfide (CdS) nanoparticle formation in yeasts and its synthesis necessitates two enzymes: \u03b3\u2010glutamylcysteine synthetase (\u03b3\u2010GCS) and glutathione synthetase (GS). Hereby, we constructed two recombinant <jats:italic>E. coli<\/jats:italic> ABLE C strains to over\u2010express either \u03b3\u2010GCS or GS and found that \u03b3\u2010GCS over\u2010expression resulted in inclusion body formation and impaired cell physiology, whereas GS over\u2010expression yielded abundant soluble proteins and barely impeded cell growth. Upon exposure of the recombinant cells to cadmium chloride and sodium sulfide, GS over\u2010expression augmented GSH synthesis and ameliorated CdS nanoparticles formation. The resultant CdS nanoparticles resembled those from the wild\u2010type cells in size (2\u20135 nm) and wurtzite structures, yet differed in dispersibility and elemental composition. The maximum particle yield attained in the recombinant <jats:italic>E. coli<\/jats:italic> was \u22482.5 times that attained in the wild\u2010type cells and considerably exceeded that achieved in yeasts. These data implicated the potential of genetic engineering approach to enhancing CdS nanoparticle biosynthesis in bacteria. Additionally, <jats:italic>E. coli<\/jats:italic>\u2010based biosynthesis offers a more energy\u2010efficient and eco\u2010friendly method as opposed to chemical processes requiring high temperature and toxic solvents. \u00a9 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009<\/jats:p>","DOI":"10.1002\/btpr.199","type":"journal-article","created":{"date-parts":[[2009,7,23]],"date-time":"2009-07-23T15:25:17Z","timestamp":1248362717000},"page":"1260-1266","source":"Crossref","is-referenced-by-count":94,"title":["Augmented biosynthesis of cadmium sulfide nanoparticles by genetically engineered <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"25","author":[{"given":"Yen\u2010Lin","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsing\u2010Yu","family":"Tuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Wen","family":"Tien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Hsin","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huang\u2010Chien","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Chen","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,10,12]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(00)01514-6"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0179-3"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4095(20020605)14:11<815::AID-ADMA815>3.0.CO;2-K"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt767"},{"key":"e_1_2_7_6_2","first-page":"012307\u20101","article-title":"Quantum computing with quantum dots on quantum linear supports","volume":"65","author":"Brown KR","year":"2002","journal-title":"Phys Rev A"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2007.08.016"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)71500-7"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/338596a0"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.09.004"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10233"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.59.1.7-14.1993"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2004.08.022"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1751-y"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1994.tb00103.x"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.121.4.1169"},{"key":"e_1_2_7_17_2","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1128\/aem.44.6.1444-1448.1982","article-title":"Cloning of a gene responsible for the biosynthesis of glutathione in Escherichia Coli B","volume":"44","author":"Murata K","year":"1982","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:BILE.0000018261.14427.e8"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(85)13073-9"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1762-8"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2005.12.009"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(97)10006-X"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)00099-J"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbtpr.199","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.199","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:05:21Z","timestamp":1760058321000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1002\/btpr.199"],"URL":"https:\/\/doi.org\/10.1002\/btpr.199","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9]]}}}