{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:33:20Z","timestamp":1786977200194,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T00:00:00Z","timestamp":1422316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00c3\u00a7\u00c3\u00a3o de Amparo \u00c3\u00a0 Pesquisa do Estado de S\u00c3\u00a3o Paulo\/Brazil (FAPESP)","award":["2010\/52521-6"],"award-info":[{"award-number":["2010\/52521-6"]}]},{"name":"Funda\u00c3\u00a7\u00c3\u00a3o de Amparo \u00c3\u00a0 Pesquisa do Estado de S\u00c3\u00a3o Paulo\/Brazil (FAPESP)","award":["2014\/03883-3"],"award-info":[{"award-number":["2014\/03883-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>\n                    This work aimed to compare the predictive capacity of empirical models, based on the uniform design utilization combined to artificial neural networks with respect to classical factorial designs in bioprocess, using as example the rabies virus replication in BHK\u201021 cells. The viral infection process parameters under study were temperature (34\u00b0C, 37\u00b0C), multiplicity of infection (0.04, 0.07, 0.1), times of infection, and harvest (24, 48, 72 hours) and the monitored output parameter was viral production. A multilevel factorial experimental design was performed for the study of this system. Fractions of this experimental approach (18, 24, 30, 36 and 42 runs), defined according uniform designs, were used as alternative for modelling through artificial neural network and thereafter an output variable optimization was carried out by means of genetic algorithm methodology. Model prediction capacities for all uniform design approaches under study were better than that found for classical factorial design approach. It was demonstrated that uniform design in combination with artificial neural network could be an efficient experimental approach for modelling complex bioprocess like viral production. For the present study case, 67% of experimental resources were saved when compared to a classical factorial design approach. In the near future, this strategy could replace the established factorial designs used in the bioprocess development activities performed within biopharmaceutical organizations because of the improvements gained in the economics of experimentation that do not sacrifice the quality of decisions. \u00a9 2015 American Institute of Chemical Engineers\n                    <jats:italic>Biotechnol. Prog.<\/jats:italic>\n                    , 31:532\u2013540, 2015\n                  <\/jats:p>","DOI":"10.1002\/btpr.2051","type":"journal-article","created":{"date-parts":[[2015,1,13]],"date-time":"2015-01-13T06:54:12Z","timestamp":1421132052000},"page":"532-540","source":"Crossref","is-referenced-by-count":5,"title":["Use of uniform designs in combination with neural networks for viral infection process development"],"prefix":"10.1002","volume":"31","author":[{"given":"La\u00eds Hara","family":"Buenno","sequence":"first","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas Universidade Estadual \u201cJ\u00falio de Mesquita Filho\u201d Campus\u2010Assis Avenida Dom Antonio 2100 19806\u2010900 Assis SP\u2013Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Celso","family":"Rocha","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas Universidade Estadual \u201cJ\u00falio de Mesquita Filho\u201d Campus\u2010Assis Avenida Dom Antonio 2100 19806\u2010900 Assis SP\u2013Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1824-3089","authenticated-orcid":false,"given":"Jaci","family":"Leme","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Pesquisa e Desenvolvimento em Imunol\u00f3gicos Veterin\u00e1rios, Instituto Butantan Av. Vital Brasil, 1500 05503\u2010900 S\u00e3o Paulo SP\u2013Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Pereira","family":"Caricati","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Pesquisa e Desenvolvimento em Imunol\u00f3gicos Veterin\u00e1rios, Instituto Butantan Av. Vital Brasil, 1500 05503\u2010900 S\u00e3o Paulo SP\u2013Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Tonso","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de C\u00e9lulas Animais, Departamento de Engenharia Qu\u00edmica, Escola Polit\u00e9cnica Universidade de S\u00e3o Paulo Av. Prof. Luciano Gualberto, trav. 3, 380 05508\u2010900 S\u00e3o Paulo SP\u2013Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eutimio Gustavo","family":"Fern\u00e1ndez N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas Universidade Estadual \u201cJ\u00falio de Mesquita Filho\u201d Campus\u2010Assis Avenida Dom Antonio 2100 19806\u2010900 Assis SP\u2013Brasil"},{"name":"Laborat\u00f3rio de C\u00e9lulas Animais, Departamento de Engenharia Qu\u00edmica, Escola Polit\u00e9cnica Universidade de S\u00e3o Paulo Av. Prof. Luciano Gualberto, trav. 3, 380 05508\u2010900 S\u00e3o Paulo SP\u2013Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,1,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3078"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1478-4505-10-18"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201000356"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2006.12.004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-7439(01)00139-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2011.05.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.20012"},{"key":"e_1_2_7_9_1","first-page":"33","volume-title":"Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology","author":"Chan L","year":"2010"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15792-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.1847"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-014-9756-6"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)00022-F"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","DOI":"10.11606\/T.87.2010.tde-30092010-084910","volume-title":"Valida\u00e7\u00e3o de bioensaios para o estudo da imunogenicidade da vacina contra raiva","author":"Fuches RM","year":"2010"},{"key":"e_1_2_7_15_1","unstructured":"The Pennsylvania State University Department of Statistics. Available at:http:\/\/sites.stat.psu.edu\/~rli\/DMCE\/UniformDesign\/. Accessed August 17 2014"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-7161(03)22006-X"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2014.01.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/00224065.1980.11980968"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-010-0430-x"},{"key":"e_1_2_7_20_1","first-page":"1","volume-title":"Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology","author":"Aunins J","year":"2010"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250570.spi106"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-11-84"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cjce.20233"},{"key":"e_1_2_7_24_1","volume-title":"Molecular Cell Biology","author":"Lodish H","year":"2008"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10532-006-9072-8"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00201-3"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.1601"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(01)00362-5"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-36213-5_6"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbtpr.2051","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.2051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:28:55Z","timestamp":1760048935000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.2051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,27]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1002\/btpr.2051"],"URL":"https:\/\/doi.org\/10.1002\/btpr.2051","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,27]]}}}