{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T11:11:29Z","timestamp":1783768289795,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,2,26]],"date-time":"2015-02-26T00:00:00Z","timestamp":1424908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Scientific and Technological Research Council of Turkey (TUBITAK)","award":["113 Z 678"],"award-info":[{"award-number":["113 Z 678"]}]},{"name":"Trakya University Scientific Research Foundation","award":["2011\/195"],"award-info":[{"award-number":["2011\/195"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>This paper presents a novel hormone\u2010based impedimetric biosensor to determine parathyroid hormone (PTH) level in serum for diagnosis and monitoring treatment of hyperparathyroidism, hypoparathyroidism and thyroid cancer. The interaction between PTH and the biosensor was investigated by an electrochemical method. The biosensor was based on the gold electrode modified by 12\u2010mercapto dodecanoic (12MDDA). Antiparathyroid hormone (anti\u2010PTH) was covalently immobilized on to poly amidoamine dendrimer (PAMAM) which was bound to a 1\u2010ethyl\u20103\u2010(3\u2010dimethylaminopropyl)\u2010carbodiimide\/N\u2010hydroxysuccinimide (EDC\/NHS) couple, self\u2010assembled monolayer structure from one of the other NH<jats:sub>2<\/jats:sub> sites. The immobilization of anti\u2010PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscope techniques. After the optimization studies of immobilization materials such as 12MDDA, EDC\u2013NHS, PAMAM, and glutaraldehyde, the performance of the biosensor was investigated in terms of linearity, sensitivity, repeatability, and reproducibility. PTH was detected within a linear range of 10\u201360 fg\/mL. Finally the described biosensor was used to monitor PTH levels in artificial serum samples. \u00a9 2015 American Institute of Chemical Engineers <jats:italic>Biotechnol. Prog<\/jats:italic>., 31:815\u2013822, 2015<\/jats:p>","DOI":"10.1002\/btpr.2060","type":"journal-article","created":{"date-parts":[[2015,2,15]],"date-time":"2015-02-15T22:37:42Z","timestamp":1424039862000},"page":"815-822","source":"Crossref","is-referenced-by-count":32,"title":["Detection of parathyroid hormone using an electrochemical impedance biosensor based on PAMAM dendrimers"],"prefix":"10.1002","volume":"31","author":[{"given":"Hakk\u0131 Mevl\u00fct","family":"\u00d6zcan","sequence":"first","affiliation":[{"name":"Chemistry Dept., Faculty of Science, University of Trakya Edirne Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Kemal","family":"Sezgint\u00fcrk","sequence":"additional","affiliation":[{"name":"Chemistry Dept., Faculty of Arts and Sciences, Nam\u0131k Kemal University Tekirda\u011f Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-5-4-403"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)85007-4"},{"key":"e_1_2_6_4_1","first-page":"15","volume-title":"The Parathyroids","author":"Brown EM","year":"1994"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2004.01.002"},{"key":"e_1_2_6_6_1","volume-title":"Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism","author":"Murray JF","year":"2003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.suc.2004.01.002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-005-0660-2"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000186337.83407.ec"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp0803050"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004080657"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr900327d"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527626953"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-2066"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/02652040802312572"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp9106052"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-009-0532-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1389-1723(04)70195-4"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/elan.200390114"},{"key":"e_1_2_6_20_1","first-page":"71","article-title":"Electrochemical techniques for the characterization of redox polymers","volume":"19","author":"Bott AW","year":"2001","journal-title":"Curr Sep"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2013.05.059"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2013.11.090"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2011.12.049"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2008.01.048"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/a606964i"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-5663(01)00277-9"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.4236\/jbise.2011.44033"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2004.08.010"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.12.034"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.04.038"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(00)01280-0"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/la960603h"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/la000035c"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2008.07.001"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.030817"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPLGCZR7IPVHA7"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.06.004"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbtpr.2060","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.2060","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:10:00Z","timestamp":1760044200000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.2060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,26]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1002\/btpr.2060"],"URL":"https:\/\/doi.org\/10.1002\/btpr.2060","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,26]]}}}