{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:27:39Z","timestamp":1791412059789,"version":"4.3.3"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,3,17]],"date-time":"2015-03-17T00:00:00Z","timestamp":1426550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>\n                    The presence of inhibitors compounds after pretreatment of lignocellulosic materials affects the saccharification and fermentation steps in bioethanol production processes. Even though, external addition of laccases selectively removes the phenolic compounds from lignocellulosic prehydrolysates, when it is coupled to saccharification step, lower hydrolysis yields are attained. Vanillin, syringaldehyde and ferulic acid are phenolic compounds commonly found in wheat\u2010straw prehydrolysate after steam\u2010explosion pretreatment. These three phenolic compounds were used in this study to elucidate the inhibitory mechanisms of laccase\u2010derived compounds after laccase treatment. Reaction products derived from laccase oxidation of vanillin and syringaldehyde showed to be the strongest inhibitors. The presence of these products causes a decrement on enzymatic hydrolysis yield of a model cellulosic substrate (Sigmacell) of 46.6 and 32.6%, respectively at 24 h. Moreover, a decrease in more than 50% of cellulase and \u03b2\u2010glucosidase activities was observed in presence of laccase and vanillin. This effect was attributed to coupling reactions between phenoxyl radicals and enzymes. On the other hand, when the hydrolysis of Sigmacell was performed in presence of prehydrolysate from steam\u2010exploded wheat straw a significant inhibition on enzymatic hydrolysis was observed independently of laccase treatment. This result pointed out that the other components of wheat\u2010straw prehydrolysate are affecting the enzymatic hydrolysis to a higher extent than the possible laccase\u2010derived products. \u00a9 2015 American Institute of Chemical Engineers\n                    <jats:italic>Biotechnol. Prog<\/jats:italic>\n                    ., 31:700\u2013706, 2015\n                  <\/jats:p>","DOI":"10.1002\/btpr.2068","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T13:26:16Z","timestamp":1425389176000},"page":"700-706","source":"Crossref","is-referenced-by-count":38,"title":["Inhibition of cellulose enzymatic hydrolysis by laccase\u2010derived compounds from phenols"],"prefix":"10.1002","volume":"31","author":[{"given":"Alfredo","family":"Oliva\u2010Taravilla","sequence":"first","affiliation":[{"name":"IMDEA Energy Inst Biotechnology Processes for Energy Production Unit Avenida Ram\u00f3n de la Sagra, 3 28935 M\u00f3stoles Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elia","family":"Tom\u00e1s\u2010Pej\u00f3","sequence":"additional","affiliation":[{"name":"IMDEA Energy Inst Biotechnology Processes for Energy Production Unit Avenida Ram\u00f3n de la Sagra, 3 28935 M\u00f3stoles Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Demuez","sequence":"additional","affiliation":[{"name":"IMDEA Energy Inst Biotechnology Processes for Energy Production Unit Avenida Ram\u00f3n de la Sagra, 3 28935 M\u00f3stoles Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Gonz\u00e1lez\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"IMDEA Energy Inst Biotechnology Processes for Energy Production Unit Avenida Ram\u00f3n de la Sagra, 3 28935 M\u00f3stoles Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Ballesteros","sequence":"additional","affiliation":[{"name":"IMDEA Energy Inst Biotechnology Processes for Energy Production Unit Avenida Ram\u00f3n de la Sagra, 3 28935 M\u00f3stoles Spain"},{"name":"CIEMAT, Renewable Energy Div Biofuels Unit Av. 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