{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:55:11Z","timestamp":1791266111283,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,9,4]],"date-time":"2015-09-04T00:00:00Z","timestamp":1441324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,4]],"date-time":"2015-09-04T00:00:00Z","timestamp":1441324800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>\n                    Anchorage\u2010dependent cell cultures are used for the production of viruses, viral vectors, and vaccines, as well as for various cell therapies and tissue engineering applications. Most of these applications currently rely on planar technologies for the generation of biological products. However, as new cell therapy product candidates move from clinical trials towards potential commercialization, planar platforms have proven to be inadequate to meet large\u2010scale manufacturing demand. Therefore, a new scalable platform for culturing anchorage\u2010dependent cells at high cell volumetric concentrations is urgently needed. One promising solution is to grow cells on microcarriers suspended in single\u2010use bioreactors. Toward this goal, a novel bioreactor system utilizing an innovative Vertical\u2010Wheel\u2122 technology was evaluated for its potential to support scalable cell culture process development. Two anchorage\u2010dependent human cell types were used: human lung carcinoma cells (A549 cell line) and human bone marrow\u2010derived mesenchymal stem cells (hMSC). Key hydrodynamic parameters such as power input, mixing time, Kolmogorov length scale, and shear stress were estimated. The performance of Vertical\u2010Wheel bioreactors (PBS\u2010VW) was then evaluated for A549 cell growth and oncolytic adenovirus type 5 production as well as for hMSC expansion. Regarding the first cell model, higher cell growth and number of infectious viruses per cell were achieved when compared with stirred tank (ST) bioreactors. For the hMSC model, although higher percentages of proliferative cells could be reached in the PBS\u2010VW compared with ST bioreactors, no significant differences in the cell volumetric concentration and expansion factor were observed. Noteworthy, the hMSC population generated in the PBS\u2010VW showed a significantly lower percentage of apoptotic cells as well as reduced levels of HLA\u2010DR positive cells. Overall, these results showed that process transfer from ST bioreactor to PBS\u2010VW, and scale\u2010up was successfully carried out for two different microcarrier\u2010based cell cultures. Ultimately, the data herein generated demonstrate the potential of Vertical\u2010Wheel bioreactors as a new scalable biomanufacturing platform for microcarrier\u2010based cell cultures of complex biopharmaceuticals. \u00a9 2015 American Institute of Chemical Engineers\n                    <jats:italic>Biotechnol. Prog.<\/jats:italic>\n                    , 31:1600\u20131612, 2015\n                  <\/jats:p>","DOI":"10.1002\/btpr.2158","type":"journal-article","created":{"date-parts":[[2015,8,20]],"date-time":"2015-08-20T03:06:06Z","timestamp":1440039966000},"page":"1600-1612","source":"Crossref","is-referenced-by-count":74,"title":["Production of oncolytic adenovirus and human mesenchymal stem cells in a single\u2010use, Vertical\u2010Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier\u2010based cell culture processes"],"prefix":"10.1002","volume":"31","author":[{"given":"Marcos F. 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