{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:49:46Z","timestamp":1788277786545,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,3,2]],"date-time":"2016-03-02T00:00:00Z","timestamp":1456876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004676","name":"CA Technologies","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004676","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>\n                    This work demonstrates a significant advance in bioprocessing for a high\u2010melting lipid polymer. A novel and environmental friendly solvent mixture, acetone\/ethanol\/propylene carbonate (A\/E\/P, 1:1:1 v\/v\/v) was identified for extracting poly\u2010hydroxybutyrate (PHB), a high\u2010value biopolymer, from Cupriavidus necator. A set of solubility curves of PHB in various solvents was established. PHB recovery of 85% and purity of 92% were obtained from defatted dry biomass (DDB) using A\/E\/P. This solvent mixture is compatible with water, and from non\u2010defatted wet biomass, PHB recovery of 83% and purity of 90% were achieved. Water and hexane were evaluated as anti\u2010solvents to assist PHB precipitation, and hexane improved recovery of PHB from biomass to 92% and the purity to 93%. A scale\u2010up extraction and separation reactor was designed, built and successfully tested. Properties of PHB recovered were not significantly affected by the extraction solvent and conditions, as shown by average molecular weight (1.4 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    ) and melting point (175.2\u00b0C) not being different from PHB extracted using chloroform. Therefore, this biorenewable solvent system was effective and versatile for extracting PHB biopolymers. \u00a9 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:678\u2013685, 2016\n                  <\/jats:p>","DOI":"10.1002\/btpr.2247","type":"journal-article","created":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T10:49:34Z","timestamp":1455274174000},"page":"678-685","source":"Crossref","is-referenced-by-count":77,"title":["Effective recovery of poly\u2010\u03b2\u2010hydroxybutyrate (PHB) biopolymer from\n                    <scp>\n                      <i>C<\/i>\n                    <\/scp>\n                    <i>upriavidus necator<\/i>\n                    using a novel and environmentally friendly solvent system"],"prefix":"10.1002","volume":"32","author":[{"given":"Tao","family":"Fei","sequence":"first","affiliation":[{"name":"Dept. of Food Science and Human Nutrition Iowa State University Ames IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy","family":"Cazeneuve","sequence":"additional","affiliation":[{"name":"Dept. of Food Science and Human Nutrition Iowa State University Ames IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyou","family":"Wen","sequence":"additional","affiliation":[{"name":"Dept. of Food Science and Human Nutrition Iowa State University Ames IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wu","sequence":"additional","affiliation":[{"name":"Dept. of Food Science and Human Nutrition Iowa State University Ames IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wang","sequence":"additional","affiliation":[{"name":"Dept. of Food Science and Human Nutrition Iowa State University Ames IA 50011"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,2]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"295","article-title":"Biosynthesis, biodegradation, and application of poly(3\u2010hydroxybutyrate) and its copolymers\u2014natural polyesters produced by diazotrophic bacteria. 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