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A green microalga with close resemblance to <jats:italic>Chlorella<\/jats:italic> sp. was found to be dominant after multiple cycles of sub\u2010culturing. Subsequently, the strain (designated as LLAI) was isolated and cultivated in 20\u00d7 diluted digester effluents under various incident light intensities (255\u20131,100 \u00b5moles m<jats:sup>\u22122<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>) to systematically assess growth and nutrient utilization. Our results showed that LLAI production increased with increasing incident light and a maximum productivity of 0.34 g L<jats:sup>\u22121<\/jats:sup> d<jats:sup>\u22121<\/jats:sup> was attained when the incident irradiance was 1,100 \u00b5moles m<jats:sup>\u22122<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>. Lack of growth in the absence of light indicated that the cultures did not grow heterotrophically on the organic compounds present in the medium. However, the cultures were able to uptake organic N and P under phototrophic conditions and our calculations suggest that the carbon associated with these organic nutrients contributed significantly to the production of biomass. Overall, under high light conditions, LLAI cultures utilized half of the soluble organic nitrogen and &gt;90% of the ammonium, orthophosphate, and dissolved organic phosphorus present in the diluted waste. Strain LLAI was also found to accumulate triacylglycerides (TAG) even before the onset of nutrient limitation and a lipid productivity of 37 mg\u2010TAG L<jats:sup>\u22121<\/jats:sup> d<jats:sup>\u22121<\/jats:sup> was measured in cultures incubated at an incident irradiance of 1,100 \u00b5moles m<jats:sup>\u22122<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>. The results of this study suggest that microalgae isolates from natural environments are well\u2010suited for nutrient remediation and biomass production from wastewater containing diverse inorganic and organic nutrient species. \u00a9 2016 American Institute of Chemical Engineers <jats:italic>Biotechnol. 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