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In this study, the suitability of ethanol and acetone for the recovery of PHB at different temperatures (from room temperature to near boiling point) in <jats:italic>Cupriavidus necator<\/jats:italic> was investigated. Experiments were performed using response surface methodology to examine the effects of different temperatures and heating incubation times on recovery percentage using the two solvents. The highest recovery percentage (92.3%) and product purity (up to 99%) were obtained with ethanol\u2010assisted extraction at 76\u00b0C for 32 min of incubation time. Under these conditions the extracted PHB exhibited a molecular mass of 1.2 \u00d7 10<jats:sup>6<\/jats:sup>. The present strategy showed that at temperatures near its boiling point, ethanol, as a nonhalogenated solvent, represents a good alternative to halogenated solvents, like chloroform, when PHB recovery is concerned. DSC analysis showed good thermal properties for ethanol\u2010 and acetone\u2010extracted biopolymers. GC and <jats:sup>1<\/jats:sup>H NMR analysis confirmed the extracted biopolymer to be polyhydroxybutyrate of good purity. \u00a9 2016 American Institute of Chemical Engineers <jats:italic>Biotechnol. 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