{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:28:11Z","timestamp":1785526091711,"version":"3.56.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T00:00:00Z","timestamp":1516147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T00:00:00Z","timestamp":1516147200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:p>\n                    Cellulose nanofibers (Cel\u2010NFs) gel can be considered as a useful drug carrier because of its biocompatibility, high specific surface area, and high loading capacity of drugs. Injectable Cel\u2010NFs gel could deliver doxorubicin (DOX) for localized chemotherapy of melanoma and suppress melanoma cells migration because of the physical barrier property of Cel\u2010NFs. We prepared DOX surface modified Cel\u2010NFs (DOX\u2010Cel\u2010NFs) gel by the electrostatic attachment of DOX molecules on the surface of Cel\u2010NFs. The increase in the zeta potential of nanofibers and the changes in the FTIR spectra of DOX\u2010Cel\u2010NFs compared to Cel\u2010NFs proved this attachment. DOX\u2010Cel\u2010NFs showed nano\u2010fibrous structure with an average diameter of 22.32\u2009\u00b1\u200910.66 nm after analyzing using field emission scanning electron microscopy. The suitable injectability of DOX\u2010Cel\u2010NFs gel verified its promising application for the localized chemotherapy. DOX\u2010Cel\u2010NFs gel exhibited a sustained drug release manner. The cytotoxicity results showed that DOX\u2010Cel\u2010NFs were more cytotoxic against melanoma cancer cells than the free DOX during 48 h incubation period. Moreover, DOX\u2010Cel\u2010NFs gel can suppress the melanoma cancer cells migration efficiently. Thus our results emphasize the potential of DOX\u2010Cel\u2010NFs gel as a chemotherapeutic agent for local delivery of DOX in order to treat melanoma and prevent its metastasis. \u00a9 2018 American Institute of Chemical Engineers\n                    <jats:italic>Biotechnol. Prog.<\/jats:italic>\n                    , 34:537\u2013545, 2018\n                  <\/jats:p>","DOI":"10.1002\/btpr.2598","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T17:51:36Z","timestamp":1515520296000},"page":"537-545","source":"Crossref","is-referenced-by-count":24,"title":["Preparation of an injectable doxorubicin surface modified cellulose nanofiber gel and evaluation of its anti\u2010tumor and anti\u2010metastasis activity in melanoma"],"prefix":"10.1002","volume":"34","author":[{"given":"Najmeh","family":"Alizadeh","sequence":"first","affiliation":[{"name":"Dept. of Pharmaceutics Novel Drug Delivery Systems Research Center, Faculty of Pharmacy, Isfahan University of Medical sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vajihe","family":"Akbari","sequence":"additional","affiliation":[{"name":"Dept. of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center Faculty of Pharmacy, Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Nurani","sequence":"additional","affiliation":[{"name":"Dept. of Pharmaceutics Novel Drug Delivery Systems Research Center, Faculty of Pharmacy, Isfahan University of Medical sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2256-040X","authenticated-orcid":false,"given":"Azade","family":"Taheri","sequence":"additional","affiliation":[{"name":"Dept. of Pharmaceutics Novel Drug Delivery Systems Research Center, Faculty of Pharmacy, Isfahan University of Medical sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,17]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/583748"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/17469872.3.5.569"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-08-0235"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/10717541003762854"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b15245"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.11.030"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.05.053"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2015.01.026"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.03.201"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.12.070"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6TB00991C"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2016.05.005"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2015.03.029"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.11.055"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.08.042"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2004.10.1160"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2015.04.063"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10570-010-9405-y"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm061215p"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10853-009-3874-0"},{"key":"e_1_2_5_22_1","first-page":"7915","volume-title":"Nanocomposites: New Trends and Developments","author":"Ebrahimi F","year":"2010"},{"key":"e_1_2_5_23_1","volume-title":"Chemistry of the Textiles Industry","author":"Carr C","year":"2012"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2012.03.031"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2012.06.011"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10570-007-9143-y"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2014.07.025"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/pj.2011.31"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2015.11.033"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2008.12.003"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(03)00468-4"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.desal.2006.03.287"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2009.12.043"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.11.020"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.3109\/03639045.2015.1058815"},{"key":"e_1_2_5_36_1","first-page":"489","article-title":"Preparation of alginate gel beads containing metformin hydrochloride using emulsion\u2010gelation method","volume":"4","author":"Choudhury PK","year":"2005","journal-title":"Trop J Pharm Res."},{"key":"e_1_2_5_37_1","first-page":"29","article-title":"Surface treatment of silica nanoparticles for stable and charge\u2010controlled colloidal silica","volume":"9","author":"Kim KM","year":"2014","journal-title":"Int J Nanomedicine."},{"key":"e_1_2_5_38_1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1515\/epoly.2004.4.1.643","article-title":"Hydrogels from chitosan crosslinked with poly (ethylene glycol) diacid as bone regeneration materials","volume":"4","author":"Mincheva R","year":"2004","journal-title":"e\u2010Polymers."},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10570-011-9546-7"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2016.01.020"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2010.01.006"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5TB01851J"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1556-276X-8-512"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-2796"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.05.018"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b08234"},{"key":"e_1_2_5_47_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1166\/mex.2015.1249","article-title":"Electrochemical impedance spectroscopic study of single\u2010stranded DNA\u2010immobilized electroactive polypyrrole\u2010coated electrospun poly (\u025b\u2010caprolactone) nanofibers","volume":"5","author":"Guler Z","year":"2015","journal-title":"Mater Express."},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4935"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.06.051"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.06.039"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/15685551.2013.771303"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1159\/000086626"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.04.039"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2008.00876.x"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00880070"},{"key":"e_1_2_5_56_1","first-page":"2169","article-title":"Three\u2010dimensional culture system as a model for studying cancer cell invasion capacity and anticancer drug sensitivity","volume":"24","author":"Doillon CJ","year":"2004","journal-title":"Anticancer Res."},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2005.10.007"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.04.047"},{"key":"e_1_2_5_59_1","volume-title":"Melanoma, An Issue of Surgical Clinics, E\u2010Book","author":"Brown KM.","year":"2014"},{"key":"e_1_2_5_60_1","volume-title":"Clinical Dermatology E\u2010Book","author":"Habif TP.","year":"2015"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbtpr.2598","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.2598","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/btpr.2598","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/btpr.2598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:26:32Z","timestamp":1773296792000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1002\/btpr.2598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,17]]},"references-count":59,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1002\/btpr.2598"],"URL":"https:\/\/doi.org\/10.1002\/btpr.2598","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"value":"8756-7938","type":"print"},{"value":"1520-6033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,17]]}}}