{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:33:15Z","timestamp":1777527195116,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T00:00:00Z","timestamp":1518393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21476210"],"award-info":[{"award-number":["21476210"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21406205"],"award-info":[{"award-number":["21406205"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:p>\n                    Halohydrin dehalogenase (HHDH)\u2010mediated dehalogenation of 1,3\u2010dichloro\u20102\u2010propanol (1,3\u2010DCP) is a key step in the chemoenzymatic synthesis of epichlorohydrin (ECH) from glycerol. In this study, a covalent immobilization strategy was employed to enhance the stability of Agrobacterium tumefaciens HHDH using epoxy resin ES\u2010103B as a carrier. Under optimal conditions, the activity recovery of ES\u2010103B\u2010immobilized HHDH (HHDH@ES\u2010103B) was 62.4% and the specific activity was 1604 U\/g. The HHDH@ES\u2010103B exhibited excellent thermostability, with a half\u2010life of 68.6 days at 40\u00b0C, which is 8.0\u2010times higher than that of the free HHDH. A semicontinuous biotransformation of 1,3\u2010DCP to ECH was performed using HHDH@ES\u2010103B as biocatalyst in a recirculating packed bed reactor (RPBR), resulting in an ECH yield of 94.2%, with an average productivity of 5.2 g\/L\/h. The RPBR system exhibited a high operational stability and even after 50 cycles of reaction, it retained\u2009&gt;\u200990% of the initial conversion. Furthermore, an integrated bioprocess based on in situ product recovery (ISPR) was developed in RPBR to overcome product inhibition. The integrated bioreactor equipped with an external macroporous adsorption resin HZD\u20109 column led to another 1.6\u2010fold increase in ECH productivity to 8.46 g\/L\/h. This improved stability and reusability of HHDH@ES\u2010103B demonstrated its potential for the biotransformation of 1,3\u2010DCP to ECH. \u00a9 2018 American Institute of Chemical Engineers\n                    <jats:italic>Biotechnol. Prog.<\/jats:italic>\n                    , 34:784\u2013792, 2018\n                  <\/jats:p>","DOI":"10.1002\/btpr.2617","type":"journal-article","created":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T04:43:15Z","timestamp":1517460195000},"page":"784-792","source":"Crossref","is-referenced-by-count":15,"title":["Covalent immobilization of halohydrin dehalogenase for efficient synthesis of epichlorohydrin in an integrated bioreactor"],"prefix":"10.1002","volume":"34","author":[{"given":"Shu\u2010Ping","family":"Zou","sequence":"first","affiliation":[{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou 310014 P.R. China"},{"name":"Engineering Research Center of Bioconversion and Biopurification of Ministry of Education Zhejiang University of Technology Hangzhou 310014 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Gu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou 310014 P.R. China"},{"name":"Engineering Research Center of Bioconversion and Biopurification of Ministry of Education Zhejiang University of Technology Hangzhou 310014 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6358-6243","authenticated-orcid":false,"given":"Yu\u2010Guo","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou 310014 P.R. 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