{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T09:16:55Z","timestamp":1788340615907,"version":"build-2803163510"},"reference-count":68,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T00:00:00Z","timestamp":1544227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2016\u20104541"],"award-info":[{"award-number":["PICT 2016\u20104541"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>\n                    <jats:italic>The electrospraying technique provides nano and microparticles that can be used as drug delivery systems. The aims of this study were, firstly, to optimize the influent parameters of electrospraying for the manufacture of a Bosentan (BOS) nanoparticulate platform, and secondly, to evaluate its physicochemical properties and<\/jats:italic>\n                    in vitro\n                    <jats:italic>biopharmaceutical behavior. Particles were characterized by scanning electron microscopy (SEM), powder X\u2010ray diffraction (PXRD), differential scanning calorimetry (DSC), thermogravimetry (TG) and Fourier transformed Infrared spectroscopy (FTIR). Drug loading, encapsulation efficiency and kinetic dissolution were determined. Additionally, Bosentan release assays at 24 and 72\u2009h were performed<\/jats:italic>\n                    in vitro\n                    <jats:italic>to evaluate biopharmaceutical properties of nano\u2010scaffolds by diffusion technique through dialysis bag. The nanostructures had heterogeneous sizes predominantly smaller than 550\u2009nm and they were semicrystalline according to PXRD, indicating a partial amorphization of BOS during the encapsulation in the polymer matrix. FT\u2010IR and DSC showed an absence of chemical interactions between BOS and \u03b5\u2010Polycaprolactone (PCL), suggesting that both components behaved as a physical mixture in these particles. The drug loading was 25.98%, and the encapsulation efficiency was 58.51%. Additionally, the release assays showed an extended and controlled release of BOS, in comparison to non\u2010encapsulated BOS. These data also showed to fit with the Cubic Root kinetic dissolution. As a conclusion, we demonstrate that the use of electrospraying for the manufacture of BOS (or similar drugs) controlled release nanoplatforms would represent an interesting contribution in the development of new therapeutic alternatives for the treatment of pathologies such as pulmonary hypertension and other related diseases<\/jats:italic>\n                    . \u00a9 2018 American Institute of Chemical Engineers\n                    <jats:italic>Biotechnol. Prog<\/jats:italic>\n                    ., 35: e2748, 2019.\n                  <\/jats:p>","DOI":"10.1002\/btpr.2748","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T05:44:41Z","timestamp":1544247881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Preparation and characterization of bosentan monohydrate\/\u03b5\u2010polycaprolactone nanoparticles obtained by electrospraying"],"prefix":"10.1002","volume":"35","author":[{"given":"Virna M.","family":"Gim\u00e9nez","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Ciencias Qu\u00edmicas. Facultad de Ciencias Qu\u00edmicas y Tecnol\u00f3gicas Universidad Cat\u00f3lica de Cuyo  San Juan Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"Sperandeo","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Farmac\u00e9uticas, Facultad de Ciencias Qu\u00edmicas Universidad Nacional de C\u00f3rdoba and UNITEFA (CONICET\u2010UNC)  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Faudone","sequence":"additional","affiliation":[{"name":"Centro de Excelencia en Productos y Procesos de C\u00f3rdoba CEPROCOR  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Noriega","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Ciencias Qu\u00edmicas. Facultad de Ciencias Qu\u00edmicas y Tecnol\u00f3gicas Universidad Cat\u00f3lica de Cuyo  San Juan Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2279-7626","authenticated-orcid":false,"given":"Walter","family":"Manucha","sequence":"additional","affiliation":[{"name":"Instituto de Medicina y Biolog\u00eda Experimental de Cuyo Consejo Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (IMBECU\u2010CONICET)  Mendoza Argentina"},{"name":"Laboratorio de Farmacolog\u00eda Experimental B\u00e1sica y Traslacional. \u00c1rea de Farmacolog\u00eda, Departamento de Patolog\u00eda, Facultad de Ciencias M\u00e9dicas Universidad Nacional de Cuyo  Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Kassuha","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Ciencias Qu\u00edmicas. 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