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However, understanding the interdependences between tissues could be of high relevance for drug target identification, especially at the first stages of disease development.\n                    <jats:italic>In vitro<\/jats:italic>\n                    systems have the advantages of accessibility to measurements and precise controllability of culture conditions, but currently have limitations in mimicking human\n                    <jats:italic>in vivo<\/jats:italic>\n                    systemic tissue response. In this work, we present an\n                    <jats:italic>in vitro<\/jats:italic>\n                    model of cross\u2010talk between an\n                    <jats:italic>ex vivo<\/jats:italic>\n                    culture of adipose tissue from an obese donor and a skeletal muscle\n                    <jats:italic>in vitro<\/jats:italic>\n                    model from a healthy donor. This is relevant to understand type 2 diabetes mellitus pathogenesis, as obesity is one of its main risk factors. The human adipose tissue biopsy was maintained as a three\u2010dimensional culture for 48\u2009h. Its conditioned culture medium was used to stimulate a human skeletal muscle\u2010on\u2010chip, developed by differentiating primary cells of a patient's biopsy under topological cues and molecular self\u2010regulation. This system has been characterized to demonstrate its ability to mimic important features of the normal skeletal muscle response\n                    <jats:italic>in vivo<\/jats:italic>\n                    . We then found that the conditioned medium from a diseased adipose tissue is able to perturb the normal insulin sensitivity of a healthy skeletal muscle, as reported in the early stages of diabetes onset. In perspective, this work represents an important step toward the development of technological platforms that allow to study and dissect the systemic interaction between unhealthy and healthy tissues\n                    <jats:italic>in vitro<\/jats:italic>\n                    . \u00a9 2018 American Institute of Chemical Engineers\n                    <jats:italic>Biotechnol. Prog<\/jats:italic>\n                    ., 35: e2766, 2019.\n                  <\/jats:p>","DOI":"10.1002\/btpr.2766","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T04:24:19Z","timestamp":1544675059000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cross\u2010talk of healthy and impaired human tissues for dissection of disease pathogenesis"],"prefix":"10.1002","volume":"35","author":[{"given":"Alice","family":"Zoso","sequence":"first","affiliation":[{"name":"Venetian Inst. of Molecular Medicine  Via Orus 2, Padova 35129 Italy"},{"name":"Dept. of Industrial Engineering University of Padova  Via Marzolo 9 Padova 35131 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Zambon","sequence":"additional","affiliation":[{"name":"Venetian Inst. of Molecular Medicine  Via Orus 2, Padova 35129 Italy"},{"name":"Dept. of Industrial Engineering 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