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Hp has various potential therapeutic applications, but it has been mainly studied for treatment of acute hemolytic conditions that can arise from situations such as massive blood transfusion, infusion of stored red blood cells, severe burns, trauma, sepsis, radiation injury, and others. Therefore, Hp may also be beneficial during chronic hemolytic disease states such as hereditary spherocytosis, nocturnal hemoglobinuria, sickle\u2010cell anemia, and malaria. Various methods have been developed to purify Hp from plasma or plasma fractions. However, none of these methods have exploited the large molecular weight (MW) range distribution of Hp polymers to easily isolate Hp from other plasma proteins. The present study used tangential flow filtration (TFF) to isolate polymeric Hp from plasma proteins using human Fraction IV (FIV) as the starting material. After removal of insoluble material from a suspension of FIV paste, the protein mixture was clarified on a 0.2 \u03bcm hollow fiber (HF) TFF filter. The clarified protein solution was then bracketed based on protein MW using HF filters with MW cut\u2010offs (MWCOs) of 750, 500, and 100\u2009kDa. Using untreated FIV, the Hp purity of the main bracket was ~75% with a total Hb binding capacity (HbBC) yield of 1.2 g starting from 500\u2009g of FIV paste. However, pretreatment of FIV with fumed silica to remove lipoproteins increased Hp purity to &gt;95% with a HbBC yield of 1.7 g per 500\u2009g of FIV. Taken together this study provides a novel and scalable method to purify Hp from plasma or plasma fractions.<\/jats:p>","DOI":"10.1002\/btpr.3010","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T15:45:36Z","timestamp":1588175136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Tangential flow filtration of haptoglobin"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4035-0027","authenticated-orcid":false,"given":"Ivan S.","family":"Pires","sequence":"first","affiliation":[{"name":"William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre F.","family":"Palmer","sequence":"additional","affiliation":[{"name":"William G. 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