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The objective of this study was to examine the use of a feed\u2010and\u2010bleed configuration to increase the effective conversion (ratio of permeate to feed flow rates) in the hollow fiber module to enable more effective washing of the precipitate. Experiments were performed using human serum Immunoglobulin G (IgG) precipitates formed with 10\u2009mM zinc chloride and 7 wt% polyethylene glycol. The critical flux was evaluated as a function of the shear rate and IgG concentration, with the resulting correlation used to predict conditions that can achieve 90% conversion in a single pass with minimal fouling. Experimental data for both the start\u2010up and steady\u2010state performance are in good agreement with model calculations. These results were used to analyze the performance of an enhanced continuous precipitation\u2013microfiltration process using the feed\u2010and\u2010bleed configuration for the initial capture \/ purification of a mAb product.<\/jats:p>","DOI":"10.1002\/btpr.3082","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T05:16:39Z","timestamp":1600319799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Enhanced filtration performance using\n                    <scp>feed\u2010and\u2010bleed<\/scp>\n                    configuration for purification of antibody precipitates"],"prefix":"10.1002","volume":"37","author":[{"given":"Zhao","family":"Li","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University, University Park  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting\u2010Hsi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University, University Park  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erha","family":"Andini","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University, University Park  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan L","family":"Coffman","sequence":"additional","affiliation":[{"name":"BioProcess Technologies and Engineering, AstraZeneca  Gaithersburg Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0111-3640","authenticated-orcid":false,"given":"Todd","family":"Przybycien","sequence":"additional","affiliation":[{"name":"Department of Chemical &amp; Biological Engineering Rensselaer Polytechnic Institute  Troy New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1865-9156","authenticated-orcid":false,"given":"Andrew L.","family":"Zydney","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University, University Park  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01207a034"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119013020.ch27"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24902"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26812"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.07.436"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr8010058"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2014.07.012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201600271"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2015.11.032"},{"key":"e_1_2_7_11_1","unstructured":"JaquezOA GronkeRS PrzybycienTM. Design of a scalable continuous precipitation process for the high throughput capture and purification of high titer monoclonal antibodies. 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