{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T22:44:22Z","timestamp":1769640262537,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008209","name":"University at Buffalo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008209","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aiche.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Yersiniabactin (Ybt) is a hybrid polyketide\u2010nonribosomal complex natural product also known as a siderophore for its iron chelation properties. The native producer of Ybt,\n                    <jats:italic>Yersinia pestis<\/jats:italic>\n                    , is a priority pathogen responsible for the plague in which the siderophore properties of Ybt are used to sequester iron and other metal species upon host infection. Alternatively, the high metal binding properties of Ybt enable a plethora of potentially valuable applications benefiting from metal remediation and\/or recovery. For these applications, a surrogate production source is highly preferred relative to the pathogenic native host. In this work, we present a modification to the heterologous\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    production system established for Ybt biosynthesis. In particular, the multiple plasmids originally used to express the genetic pathway required for Ybt biosynthesis were consolidated to a single, copy\u2010amplifiable plasmid. In so doing, plasmid stability was improved from ~30% to \u226580% while production values maintained at 20\u201330% of the original system, which resulted in titers of 0.5\u20133 mg\/L from shake flask vessels.\n                  <\/jats:p>","DOI":"10.1002\/btpr.3103","type":"journal-article","created":{"date-parts":[[2020,11,14]],"date-time":"2020-11-14T21:49:06Z","timestamp":1605390546000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Consolidated plasmid Design for Stabilized Heterologous Production of the complex natural product Siderophore Yersiniabactin"],"prefix":"10.1002","volume":"37","author":[{"given":"Ruiquan","family":"Qi","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering University at Buffalo, The State University of New York  New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Girish","family":"Swayambhu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University at Buffalo, The State University of New York  New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Bruno","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University at Buffalo, The State University of New York  New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guojian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University at Buffalo, The State University of New York  New York USA"},{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education School of Medicine and Pharmacy, Ocean University of China  Qingdao People's Republic of China"},{"name":"Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology  Qingdao People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9338-2794","authenticated-orcid":false,"given":"Blaine A.","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering University at Buffalo, The State University of New York  New York USA"},{"name":"Key Laboratory of Marine Drugs, Chinese Ministry of Education School of Medicine and Pharmacy, Ocean University of China  Qingdao People's Republic of China"},{"name":"Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology  Qingdao People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0NP00037J"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8NP00091C"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3np70034h"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2016.02.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3375-4_8"},{"issue":"5509","key":"e_1_2_9_7_1","first-page":"1790","article-title":"Biosynthesis of complex polyketides in a metabolically engineered strain of E","volume":"291","author":"Pfeifer BA","year":"2001","journal-title":"coli Science"},{"issue":"11","key":"e_1_2_9_8_1","first-page":"1232","article-title":"Complete biosynthesis of erythromycin a and designed analogs using E","volume":"17","author":"Zhang H","year":"2010","journal-title":"coli as a Heterologous Host Chem Biol"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2005.08.001"},{"key":"e_1_2_9_10_1","first-page":"86","volume-title":"The chemistry and biology of fatty acid, polyketide, and nonribosomal peptide biosynthesis","author":"Carreras C","year":"1997"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-9-2159"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.11.6698-6702.2003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(02)00115-1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-145-5-1181"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.04.008"},{"issue":"6","key":"e_1_2_9_16_1","first-page":"1011","article-title":"Metal selectivity by the virulence\u2010associated yersiniabactin metallophore system. 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